An MEKC assay for the therapeutic drug monitoring of cefepime

Theurillat, Regula; Sendi, Parham; Thormann, Wolfgang (2013). An MEKC assay for the therapeutic drug monitoring of cefepime. Journal of separation science, 36(17), pp. 2915-2921. Wiley-VCH 10.1002/jssc.201300402

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The development of a robust assay based on MEKC for cefepime in human serum and plasma with internal quality assurance is reported. Sample preparation comprises protein precipitation in the presence of SDS at pH 4.5. This is a gentle approach for which decomposition of cefepime during sample handling is negligible. After hydrodynamic sample injection of the supernatant, analysis occurs in a phosphate/borate buffer at pH 9.1 with 75 mM SDS using normal polarity and analyte detection at 257 nm. The MEKC run time interval and throughput are about 5 min and seven samples per hour, respectively. The calibration range for cefepime is 1-60 μg/mL, with 1 μg/mL being the LOQ. The performance of the assay with multilevel internal calibration was assessed with calibration and control samples. The assay is shown to be simple, inexpensive, reproducible, and robust. It was applied to determine cefepime levels in the sera of critically ill patients and to assess the instability of cefepime in patient and control samples. Our data revealed that serum containing cefepime can be stored at -20°C for a short time, whereas for long-term storage, samples have to be kept at -70°C.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Laboratory for Clinical Pharmacology
04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Infectiology

UniBE Contributor:

Theurillat, Regula; Sendi, Parham and Thormann, Wolfgang

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1615-9306

Publisher:

Wiley-VCH

Language:

English

Submitter:

Annelies Luginbühl

Date Deposited:

21 Mar 2014 09:08

Last Modified:

03 Mar 2017 18:07

Publisher DOI:

10.1002/jssc.201300402

PubMed ID:

23857909

Uncontrolled Keywords:

CE, Cefepime, MEKC, Therapeutic drug monitoring

BORIS DOI:

10.7892/boris.44310

URI:

https://boris.unibe.ch/id/eprint/44310

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