Use of a Th1 Stimulator Adjuvant for Vaccination against Neospora caninum Infection in the Pregnant Mouse Model

Monney, Thierry; Grandgirard, Denis; Leib, Stephen; Hemphill, Andrew (2013). Use of a Th1 Stimulator Adjuvant for Vaccination against Neospora caninum Infection in the Pregnant Mouse Model. Pathogens, 2(2), pp. 193-208. MDPI AG 10.3390/pathogens2020193

[img]
Preview
Text
pdf.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (392kB) | Preview

Vertical transmission from an infected cow to its fetus accounts for the vast majority of new Neospora caninum infections in cattle. A vaccine composed of a chimeric antigen named recNcMIC3-1-R, based on predicted immunogenic domains of the two microneme proteins NcMIC1 and NcMIC3, the rhoptry protein NcROP2, and emulsified in saponin adjuvants, significantly reduced the cerebral infection in non-pregnant BALB/c mice. Protection was associated with a mixed Th1/Th2-type cytokine response. However, the same vaccine formulation elicited a Th2-type immune response in pregnant mice and did not prevent vertical transmission or disease, neither in dams nor in offspring mice. In this study, an alternative vaccine formulation containing recNcMIC3-1-R emulsified in Freund’s incomplete adjuvant, a stimulator of the cellular immunity, was investigated. No protection against vertical transmission and cerebral infection in the pregnant mice and a very limited protective effect in the non-pregnant mice were observed. The vaccine induced a Th1-type immune response characterized by high IgG2a titres and strong IFN-γ expression, which appeared detrimental to pregnancy.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases
05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Monney, Thierry; Grandgirard, Denis; Leib, Stephen and Hemphill, Andrew

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health
600 Technology > 630 Agriculture

ISSN:

2076-0817

Publisher:

MDPI AG

Funders:

[4] Swiss National Science Foundation

Language:

English

Submitter:

Susanne Portner

Date Deposited:

26 May 2014 09:20

Last Modified:

08 Feb 2017 20:36

Publisher DOI:

10.3390/pathogens2020193

BORIS DOI:

10.7892/boris.44746

URI:

https://boris.unibe.ch/id/eprint/44746

Actions (login required)

Edit item Edit item
Provide Feedback