RNA-dependent genome processing during nuclear differentiation: the model systems of stichotrichous ciliates

Fuhrmann, Gloria; Swart, Estienne; Nowacki, Mariusz; Lipps, Hans J. (2013). RNA-dependent genome processing during nuclear differentiation: the model systems of stichotrichous ciliates. Epigenomics, 5(2), pp. 229-236. Future Medicine 10.2217/epi.13.15

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We introduce ciliated protozoa, and more specifically the stichotrichous ciliates Oxytricha and Stylonychia, as biological model systems for the analysis of programmed DNA-reorganization processes during nuclear differentiation. These include DNA excision, DNA elimination, reordering of gene segments and specific gene amplification. We show that small nuclear RNAs specify DNA sequences to be excised or retained, but also discuss the need for a RNA template molecule derived from the parental nucleus for these processes. This RNA template guides reordering of gene segments to become functional genes and determines gene copy number in the differentiated nucleus. Since the template is derived from the parental macronucleus, gene reordering and DNA amplification are inherited in a non-Mendelian epigenetic manner.

Item Type:

Journal Article (Further Contribution)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology
08 Faculty of Science > Other Institutions > Teaching Staff, Faculty of Science
08 Faculty of Science > Department of Biology > Institute of Cell Biology > Ciliate

UniBE Contributor:

Swart, Estienne Carl and Nowacki, Mariusz

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

1750-1911

Publisher:

Future Medicine

Language:

English

Submitter:

Mariusz Nowacki

Date Deposited:

24 Apr 2014 08:41

Last Modified:

24 Apr 2014 08:41

Publisher DOI:

10.2217/epi.13.15

PubMed ID:

23566098

URI:

https://boris.unibe.ch/id/eprint/45230

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