Microscopic analysis of chromatin localization and dynamics in C. elegans

Lanctôt, Christian; Meister, Pierre (2013). Microscopic analysis of chromatin localization and dynamics in C. elegans. In: Shav-Tal, Yaron (ed.) Imaging Gene Expression. Methods in Molecular Biology: Vol. 1042 (pp. 153-172). New York: Humana Press 10.1007/978-1-62703-526-2_11

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During development, the genome undergoes drastic reorganization within the nuclear space. To determine tridimensional genome folding, genome-wide techniques (damID/Hi-C) can be applied using cell populations, but these have to be calibrated using microscopy and single-cell analysis of gene positioning. Moreover, the dynamic behavior of chromatin has to be assessed on living samples. Combining fast stereotypic development with easy genetics and microscopy, the nematode C. elegans has become a model of choice in recent years to study changes in nuclear organization during cell fate acquisition. Here we present two complementary techniques to evaluate nuclear positioning of genes either by fluorescence in situ hybridization in fixed samples or in living worm embryos using the GFP-lacI/lacO chromatin-tagging system.

Item Type:

Book Section (Book Chapter)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology

UniBE Contributor:

Meister, Pierre

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

1064-3745

ISBN:

978-1-62703-525-5

Series:

Methods in Molecular Biology

Publisher:

Humana Press

Language:

English

Submitter:

Pierre Meister

Date Deposited:

04 Aug 2014 17:15

Last Modified:

25 Dec 2014 18:40

Publisher DOI:

10.1007/978-1-62703-526-2_11

PubMed ID:

23980006

Uncontrolled Keywords:

Nuclear organization, C. elegans, Fluorescence in situ hybridization, Chromatin tagging, GFP-lacI/lacO, Microscopy

BORIS DOI:

10.7892/boris.45435

URI:

https://boris.unibe.ch/id/eprint/45435

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