Promoter- and RNA polymerase II-dependent hsp-16 gene association with nuclear pores in Caenorhabditis elegans.

Rohner, Sabine; Kalck, Veronique; Wang, Xuefei; Ikegami, Kohta; Lieb, Jason D.; Gasser, Susan M.; Meister, Peter; Meister, Pierre (2013). Promoter- and RNA polymerase II-dependent hsp-16 gene association with nuclear pores in Caenorhabditis elegans. Journal of cell biology, 200(5), pp. 589-604. Rockefeller Institute Press 10.1083/jcb.201207024

[img]
Preview
Text
Rohner_JCB_2013.pdf - Published Version
Available under License Publisher holds Copyright.

Download (3MB) | Preview

Some inducible yeast genes relocate to nuclear pores upon activation, but the general relevance of this phenomenon has remained largely unexplored. Here we show that the bidirectional hsp-16.2/41 promoter interacts with the nuclear pore complex upon activation by heat shock in the nematode Caenorhabditis elegans. Direct pore association was confirmed by both super-resolution microscopy and chromatin immunoprecipitation. The hsp-16.2 promoter was sufficient to mediate perinuclear positioning under basal level conditions of expression, both in integrated transgenes carrying from 1 to 74 copies of the promoter and in a single-copy genomic insertion. Perinuclear localization of the uninduced gene depended on promoter elements essential for induction and required the heat-shock transcription factor HSF-1, RNA polymerase II, and ENY-2, a factor that binds both SAGA and the THO/TREX mRNA export complex. After induction, colocalization with nuclear pores increased significantly at the promoter and along the coding sequence, dependent on the same promoter-associated factors, including active RNA polymerase II, and correlated with nascent transcripts.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology

UniBE Contributor:

Meister, Pierre

Subjects:

500 Science > 570 Life sciences; biology
500 Science

ISSN:

0021-9525

Publisher:

Rockefeller Institute Press

Language:

English

Submitter:

Pierre Meister

Date Deposited:

07 Aug 2014 13:48

Last Modified:

13 Dec 2014 06:15

Publisher DOI:

10.1083/jcb.201207024

PubMed ID:

23460676

BORIS DOI:

10.7892/boris.45437

URI:

https://boris.unibe.ch/id/eprint/45437

Actions (login required)

Edit item Edit item
Provide Feedback