Temporal and Spatial Coexistence of Archaeal and Bacterial amoA Genes and Gene Transcripts in Lake Lucerne

Vissers, Elisabeth W.; Anselmetti, Flavio S.; Bodelier, Paul L. E.; Muyzer, Gerard; Schleper, Christa; Tourna, Maria; Laanbroek, Hendrikus J. (2013). Temporal and Spatial Coexistence of Archaeal and Bacterial amoA Genes and Gene Transcripts in Lake Lucerne. Archaea, 2013(289478), pp. 1-11. Heron Pub. 10.1155/2013/289478

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Despite their crucial role in the nitrogen cycle, freshwater ecosystems are relatively rarely studied for active ammonia oxidizers (AO). This study of Lake Lucerne determined the abundance of both amoA genes and gene transcripts of ammonia-oxidizing archaea (AOA) and bacteria (AOB) over a period of 16 months, shedding more light on the role of both AO in a deep, alpine lake environment. At the surface, at 42 m water depth, and in the water layer immediately above the sediment, AOA generally outnumbered AOB. However, in the surface water during summer stratification, when both AO were low in abundance, AOB were more numerous than AOA. Temporal distribution patterns of AOA and AOB were comparable. Higher abundances of amoA gene transcripts were observed at the onset and end of summer stratification. In summer, archaeal amoA genes and transcripts correlated negatively with temperature and conductivity. Concentrations of ammonium and oxygen did not vary enough to explain the amoA gene and transcript dynamics. The observed herbivorous zooplankton may have caused a hidden flux of mineralized ammonium and a change in abundance of genes and transcripts. At the surface, AO might have been repressed during summer stratification due to nutrient limitation caused by active phytoplankton.

Item Type:

Journal Article (Original Article)

Division/Institute:

10 Strategic Research Centers > Oeschger Centre for Climate Change Research (OCCR)
08 Faculty of Science > Institute of Geological Sciences
08 Faculty of Science > Institute of Geological Sciences > Quaternary Geology

UniBE Contributor:

Anselmetti, Flavio

Subjects:

500 Science > 550 Earth sciences & geology

ISSN:

1472-3646

Publisher:

Heron Pub.

Language:

English

Submitter:

Flavio Anselmetti

Date Deposited:

13 Aug 2014 09:40

Last Modified:

26 Jun 2016 01:49

Publisher DOI:

10.1155/2013/289478

BORIS DOI:

10.7892/boris.45623

URI:

https://boris.unibe.ch/id/eprint/45623

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