Bovine Staphylococcus aureus: diagnostic properties of specific media

Graber, Hans Ulrich; Pfister, S.; Burgener, Pascale; Boss, Renate; Meylan, Mireille; Hummerjohann, J. (2013). Bovine Staphylococcus aureus: diagnostic properties of specific media. Research in veterinary science, 95(1), pp. 38-44. Association for Veterinary Teaching and Research Work 10.1016/j.rvsc.2013.02.023

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As accurate discrimination between Staphylococcus (S.) aureus and NSA (non-S. aureus staphylococci) involved in bovine mastitis is essential in terms of clinical prognosis and outcome, the aim of this study was to reevaluate the classical bacteriological procedures to identify these agents. Various media and the coagulase tube test were investigated using 116 strains of S. aureus and 115 of NSA, all isolated from cows with spontaneous intramammary infections (IMI). Furthermore, 25 NSA reference strains were analyzed. The study demonstrated that a few media were appropriate for differentiating S. aureus from NSA, provided that the staphylococci were isolated from bovine IMI. Evaluation of hemolysis further revealed that double or incomplete hemolysis are specific for S. aureus and are, therefore, a decisive diagnostic criterion. For strains showing complete hemolysis, maximal discrimination between S. aureus and NSA was observed by subculturing them on CHROMagar Staph. aureus.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Veterinary Public Health / Herd Health Management
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Clinic for Ruminants

UniBE Contributor:

Graber, Hans Ulrich; Burgener, Pascale; Boss, Renate and Meylan, Mireille

Subjects:

600 Technology > 630 Agriculture

ISSN:

0034-5288

Publisher:

Association for Veterinary Teaching and Research Work

Language:

English

Submitter:

Susanne Portner

Date Deposited:

22 Aug 2014 14:41

Last Modified:

14 Oct 2015 11:32

Publisher DOI:

10.1016/j.rvsc.2013.02.023

PubMed ID:

23548479

Uncontrolled Keywords:

Cattle, Mastitis, Staphylococcus aureus, Phenotyping, PCR, Diagnostics

BORIS DOI:

10.7892/boris.45817

URI:

https://boris.unibe.ch/id/eprint/45817

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