Diagnostic gap in Bovine viral diarrhea virus serology during the periparturient period in cattle

Bachofen, Claudia; Bollinger, Barbara; Peterhans, Ernst; Stalder, Hanspeter; Schweizer, Matthias (2013). Diagnostic gap in Bovine viral diarrhea virus serology during the periparturient period in cattle. Journal of veterinary diagnostic investigation, 25(5), pp. 655-661. American Association of Veterinary Laboratory Diagnosticians AAVLD 10.1177/1040638713501172

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Detection of antibodies against Bovine viral diarrhea virus (BVDV) in serum and milk by enzyme-linked immunosorbent assay (ELISA) is a crucial part of all ongoing national schemes to eradicate this important cattle pathogen. Serum and milk are regarded as equally suited for antibody measurement. However, when retesting a seropositive cow 1 day after calving, the serum was negative in 6 out of 9 different ELISAs. To further investigate this diagnostic gap around parturition, pre- and postcalving serum and milk samples of 5 cows were analyzed by BVDV antibody ELISA and serum neutralization test (SNT). By ELISA, 3 out of the 5 animals showed a diagnostic gap in the serum for up to 12 days around calving but all animals remained positive in SNT. In milk, the ELISA was strongly positive after birth but antibody levels decreased considerably within the next few days. Because of the immunoglobulin G (IgG)1-specific transport of serum antibodies into the mammary gland for colostrum production, the IgG subclass specificity of the total and the BVDV-specific antibodies were determined. Although all 5 animals showed a clear decrease in the total and BVDV-specific IgG1 antibody levels at parturition, the precalving IgG1-to-IgG2 ratios of the BVDV-specific antibodies were considerably lower in animals that showed the diagnostic gap. Results showed that BVDV seropositive cows may become "false" negative in several ELISAs in the periparturient period and suggest that the occurrence of this diagnostic gap is influenced by the BVDV-specific IgG subclass response of the individual animal.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Bachofen, Claudia; Bollinger, Barbara; Peterhans, Ernst; Stalder, Hanspeter and Schweizer, Matthias

Subjects:

600 Technology > 630 Agriculture

ISSN:

1040-6387

Publisher:

American Association of Veterinary Laboratory Diagnosticians AAVLD

Language:

English

Submitter:

Susanne Portner

Date Deposited:

22 Aug 2014 14:34

Last Modified:

10 Feb 2015 09:12

Publisher DOI:

10.1177/1040638713501172

PubMed ID:

24029404

Uncontrolled Keywords:

Bovine viral diarrhea virus, calving, colostrum, diagnostic gap, enzyme-linked immunosorbent assay, periparturient period, serology

BORIS DOI:

10.7892/boris.45843

URI:

https://boris.unibe.ch/id/eprint/45843

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