Hilbe, M.; Girao, V.; Bachofen, Claudia; Schweizer, Matthias; Zlinszky, K.; Ehrensperger, F. (2013). Apoptosis in Bovine viral diarrhea virus (BVDV)-induced mucosal disease lesions: a histological, immunohistological, and virological investigation. Veterinary pathology, 50(1), pp. 46-55. American College of Veterinary Pathologists 10.1177/0300985812447826
Full text not available from this repository.Cattle persistently infected with a noncytopathic Bovine viral diarrhea virus (BVDV) are at risk of developing fatal "mucosal disease" (MD). The authors investigated the role of various apoptosis pathways in the pathogenesis of lesions in animals suffering from MD. Therefore, they compared the expression of caspase-3, caspase-8, caspase-9, and Bcl-2L1 (Bcl-x) in tissues of 6 BVDV-free control animals, 7 persistently infected (PI) animals that showed no signs of MD (non-MD PI animals), and 11 animals with MD and correlated the staining with the localization of mucosal lesions. Caspase-3 and -9 staining were markedly stronger in MD cases and were associated with mucosal lesions, even though non-MD PI animals and negative controls also expressed caspase-9. Conversely, caspase-8 was not elevated in any of the animals analyzed. Interestingly, Bcl-x also colocalized with mucosal lesions in the MD cases. However, Bcl-x was similarly expressed in tissues from all 3 groups, and thus, its role in apoptosis needs to be clarified. This study clearly illustrates ex vivo that the activation of the intrinsic, but not the extrinsic, apoptosis pathway is a key element in the pathogenesis of MD lesions observed in cattle persistently infected with BVDV. However, whether direct induction of apoptosis in infected cells or indirect effects induced by the virus are responsible for the lesions observed remains to be established.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) |
UniBE Contributor: |
Bachofen, Claudia, Schweizer, Matthias |
Subjects: |
600 Technology > 630 Agriculture |
ISSN: |
0300-9858 |
Publisher: |
American College of Veterinary Pathologists |
Language: |
English |
Submitter: |
Susanne Portner |
Date Deposited: |
28 Jul 2014 13:37 |
Last Modified: |
05 Dec 2022 14:31 |
Publisher DOI: |
10.1177/0300985812447826 |
PubMed ID: |
22700847 |
Uncontrolled Keywords: |
apoptosis, caspases, Bcl-x, Bovine viral diarrhea virus (BVDV), cytopathic, noncytopathic, immunohistochemistry, mucosal disease |
URI: |
https://boris.unibe.ch/id/eprint/45942 |
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