Apoptosis in Bovine viral diarrhea virus (BVDV)-induced mucosal disease lesions: a histological, immunohistological, and virological investigation

Hilbe, M.; Girao, V.; Bachofen, Claudia; Schweizer, Matthias; Zlinszky, K.; Ehrensperger, F. (2013). Apoptosis in Bovine viral diarrhea virus (BVDV)-induced mucosal disease lesions: a histological, immunohistological, and virological investigation. Veterinary pathology, 50(1), pp. 46-55. American College of Veterinary Pathologists 10.1177/0300985812447826

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Cattle persistently infected with a noncytopathic Bovine viral diarrhea virus (BVDV) are at risk of developing fatal "mucosal disease" (MD). The authors investigated the role of various apoptosis pathways in the pathogenesis of lesions in animals suffering from MD. Therefore, they compared the expression of caspase-3, caspase-8, caspase-9, and Bcl-2L1 (Bcl-x) in tissues of 6 BVDV-free control animals, 7 persistently infected (PI) animals that showed no signs of MD (non-MD PI animals), and 11 animals with MD and correlated the staining with the localization of mucosal lesions. Caspase-3 and -9 staining were markedly stronger in MD cases and were associated with mucosal lesions, even though non-MD PI animals and negative controls also expressed caspase-9. Conversely, caspase-8 was not elevated in any of the animals analyzed. Interestingly, Bcl-x also colocalized with mucosal lesions in the MD cases. However, Bcl-x was similarly expressed in tissues from all 3 groups, and thus, its role in apoptosis needs to be clarified. This study clearly illustrates ex vivo that the activation of the intrinsic, but not the extrinsic, apoptosis pathway is a key element in the pathogenesis of MD lesions observed in cattle persistently infected with BVDV. However, whether direct induction of apoptosis in infected cells or indirect effects induced by the virus are responsible for the lesions observed remains to be established.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Bachofen, Claudia and Schweizer, Matthias

Subjects:

600 Technology > 630 Agriculture

ISSN:

0300-9858

Publisher:

American College of Veterinary Pathologists

Language:

English

Submitter:

Susanne Portner

Date Deposited:

28 Jul 2014 13:37

Last Modified:

14 Oct 2015 11:37

Publisher DOI:

10.1177/0300985812447826

PubMed ID:

22700847

Uncontrolled Keywords:

apoptosis, caspases, Bcl-x, Bovine viral diarrhea virus (BVDV), cytopathic, noncytopathic, immunohistochemistry, mucosal disease

URI:

https://boris.unibe.ch/id/eprint/45942

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