miR-21 and its target gene CCL20 are both highly overexpressed in the microenvironment of colorectal tumors: significance of their regulation

Vicinus, Benjamin; Rubie, Claudia; Stegmaier, Nathalie; Frick, Vilma Oliveira; Kölsch, Kathrin; Kauffels, Anne; Ghadjar, Pirus; Wagner, Mathias; Glanemann, Matthias (2013). miR-21 and its target gene CCL20 are both highly overexpressed in the microenvironment of colorectal tumors: significance of their regulation. Oncology reports, 30(3), pp. 1285-1292. Spandidos Publications 10.3892/or.2013.2580

Full text not available from this repository. (Request a copy)

Recently, we reported a functional interaction between miR-21 and its identified chemokine target CCL20 in colorectal cancer (CRC) cell lines. Here, we investigated whether such functional interactions are permitted at the cellular level which would require an inverse correlation of expression and also co-expression of miR-21 and CCL20 in the same cell. Expression profiling was performed using qPCR, and ELISA, in situ hybridization and immunohistochemistry were applied for the presentation of their cellular localization. We demonstrated that miR-21 as well as CCL20 were both significantly upregulated in CRC tissues; thus, showing no antidromic expression pattern. This provided an initial clue that miR-21 and CCL20 may not be expressed in the same cell. In addition, we located miR-21 expression at the cellular level predominantly in stromal cells such as tumor-associated fibroblasts and to a minor degree in immune cells such as macrophages and lymphocytes. Likewise, CCL20 expression was primarily detected in tumor-infiltrating immune cells. Thus, investigating the cellular localization of miR-21 and its target CCL20 revealed that both molecules are expressed predominantly in the microenvironment of CRC tumors.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Radiation Oncology

UniBE Contributor:

Ghadjar, Pirus

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1021-335X

Publisher:

Spandidos Publications

Language:

English

Submitter:

Beatrice Scheidegger

Date Deposited:

24 Apr 2014 09:07

Last Modified:

24 Apr 2014 09:07

Publisher DOI:

10.3892/or.2013.2580

PubMed ID:

23817679

URI:

https://boris.unibe.ch/id/eprint/46012

Actions (login required)

Edit item Edit item
Provide Feedback