Confocal imaging protocols for live/dead staining in three-dimensional carriers

Gantenbein-Ritter, Benjamin; Sprecher, Christoph M.; Chan, Samantha; Illien-Junger, Svenja; Grad, Sibylle (2011). Confocal imaging protocols for live/dead staining in three-dimensional carriers. Methods in molecular biology, 740, pp. 127-140. Totowa, N.J.: Humana Press 10.1007/978-1-61779-108-6_14

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In tissue engineering, a variety of methods are commonly used to evaluate survival of cells inside tissues or three-dimensional (3D) carriers. Among these methods confocal laser scanning microscopy opened accessibility of 3D tissue using live cell imaging into the tissue or 3D scaffolds. However, although this technique is ideally applied to 3D tissue or scaffolds with thickness up to several millimetres, this application is surprisingly rare and scans are often done on slices with thickness <20 μm. Here, we present novel protocols for the staining of 3D tissue (e.g. intervertebral disc tissue) and scaffolds, such as fibrin gels or alginate beads.

Item Type:

Journal Article (Original Article)

Division/Institute:

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04 Faculty of Medicine > Pre-clinic Human Medicine > Institute for Surgical Technology & Biomechanics ISTB

UniBE Contributor:

Gantenbein, Benjamin and Chan, Samantha

ISSN:

1064-3745

Publisher:

Humana Press

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:16

Last Modified:

26 Feb 2016 14:50

Publisher DOI:

10.1007/978-1-61779-108-6_14

PubMed ID:

21468974

URI:

https://boris.unibe.ch/id/eprint/4654 (FactScience: 209203)

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