Glucocorticoid induced impairment of lung structure assessed by digital image analysis

Tschanz, Stefan A.; Haenni, B.; Burri, P. H. (2000). Glucocorticoid induced impairment of lung structure assessed by digital image analysis. European journal of pediatrics, 161(1), pp. 26-30. Springer 10.1007/s00431-001-0852-1

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Glucocorticoids (GC) are successfully applied in neonatology to improve lung maturation in preterm born babies. Animal studies show that GC can also impair lung development. In this investigation, we used a new approach based on digital image analysis. Microscopic images of lung parenchyma were skeletonised and the geometrical properties of the septal network characterised by analysing the 'skeletal' parameters. Inhibition of the process of alveolarisation after extensive administration of small doses of GC in newborn rats was confirmed by significant changes in the 'skeletal' parameters. The induced structural changes in the lung parenchyma were still present after 60 days in adult rats, clearly indicating a long lasting or even definitive impairment of lung development and maturation caused by GC. Conclusion: digital image analysis and skeletonisation proved to be a highly suited approach to assess structural changes in lung parenchyma.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy

UniBE Contributor:

Tschanz, Stefan A.; Haenni, Beat and Burri, Peter Hermann

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0340-6199

Publisher:

Springer

Language:

English

Submitter:

Stefan Andreas Tschanz

Date Deposited:

25 Aug 2014 17:05

Last Modified:

20 Dec 2016 15:31

Publisher DOI:

10.1007/s00431-001-0852-1

PubMed ID:

11808877

Uncontrolled Keywords:

Digital image analysis, Glucocorticoids, Lung, Microscopy, Morphometry

BORIS DOI:

10.7892/boris.46875

URI:

https://boris.unibe.ch/id/eprint/46875

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