Nano Aerosol Chamber for In-Vitro Toxicity (NACIVT) studies.

Jeannet, Natalie; Fierz, Martin; Kalberer, Markus; Burtscher, Heinz; Geiser, Marianne (2014). Nano Aerosol Chamber for In-Vitro Toxicity (NACIVT) studies. Nanotoxicology, 9(1), pp. 34-42. Informa Healthcare 10.3109/17435390.2014.886739

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Abstract Inhalation of ambient air particles or engineered nanoparticles (NP) handled as powders, dispersions or sprays in industrial processes and contained in consumer products pose a potential and largely unknown risk for incidental exposure. For efficient, economical and ethically sound evaluation of health hazards by inhaled nanomaterials, animal-free and realistic in vitro test systems are desirable. The new Nano Aerosol Chamber for in-vitro Toxicity studies (NACIVT) has been developed and fully characterized regarding its performance. NACIVT features a computer-controlled temperature and humidity conditioning, preventing cellular stress during exposure and allowing long-term exposures. Airborne NP are deposited out of a continuous air stream simultaneously on up to 24 cell cultures on Transwell® inserts, allowing high-throughput screening. In NACIVT, polystyrene as well as silver particles were deposited uniformly and efficiently on all 24 Transwell® inserts. Particle-cell interaction studies confirmed that deposited particles reach the cell surface and can be taken up by cells. As demonstrated in control experiments, there was no evidence for any adverse effects on human bronchial epithelial cells (BEAS-2B) due to the exposure treatment in NACIVT. The new, fully integrated and transportable deposition chamber NACIVT provides a promising tool for reliable, acute and sub-acute dose-response studies of (nano)particles in air-exposed tissues cultured at the air-liquid interface.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy

UniBE Contributor:

Jeannet, Natalie and Geiser, Marianne

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1743-5390

Publisher:

Informa Healthcare

Language:

English

Submitter:

Natalie Jeannet

Date Deposited:

26 Sep 2014 16:22

Last Modified:

26 Jun 2016 01:49

Publisher DOI:

10.3109/17435390.2014.886739

PubMed ID:

24552156

URI:

https://boris.unibe.ch/id/eprint/46967

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