Dental Caries and Microbiota in Children with Black Stain and Non-discoloured Dental Plaque

Heinrich-Weltzien, R.; Bartsch, B.; Eick, Sigrun (2014). Dental Caries and Microbiota in Children with Black Stain and Non-discoloured Dental Plaque. Caries research, 48(2), pp. 118-125. Karger 10.1159/000353469

[img] Text
2014_CarieRes.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (174kB) | Request a copy
[img]
Preview
Text
Revision_2-Black stain_27-05-2013.pdf - Accepted Version
Available under License Publisher holds Copyright.

Download (204kB) | Preview

Aim: We aimed to assess caries experience and microbiota in systemically healthy children with black stain (BS) and non-discoloured plaque. Methods: Forty-six children with BS and 47 counterparts with non-discoloured plaque aged 7.9 ± 1.3 years were clinically examined. Dental caries was scored using WHO criteria. Samples of BS and non-discoloured dental plaque were collected from tooth surfaces. The DNA of the samples was extracted and real-time PCR was performed to determine the total number of bacteria and the species Streptococcus mutans, S. sobrinus, Lactobacillus sp., Actinomyces naeslundii, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia and Fusobacterium nucleatum. Results: Children with BS had lower DMFT (p = 0.013), lower DT values (p = 0.005) and a tendency to lower caries prevalence (p = 0.061) than children with non-discoloured plaque. Plaque samples of the BS group contained higher numbers of A. naeslundii (p = 0.005) and lower numbers of F. nucleatum (p = 0.001) and Lactobacillus sp. (p = 0.001) compared to the non-discoloured plaque samples of the control group. Comparing the children with BS and non-discoloured plaque, higher counts for A. naeslundii (p = 0.013) were observed in caries-free children with BS while in caries-affected children with BS, lower counts of F. nucleatum (p = 0.007) were found. Counts of Lactobacillus sp. were higher in non-discoloured plaque samples than in BS of caries-free and caries-affected children. Conclusion: Results suggest that the different microbial composition of BS might be associated with the lower caries experience in affected subjects. The role of black-pigmented bacteria associated with periodontitis needs further studies. © 2013 S. Karger AG, Basel.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology

UniBE Contributor:

Eick, Sigrun

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0008-6568

Publisher:

Karger

Language:

English

Submitter:

Eveline Carmen Schuler

Date Deposited:

01 May 2014 09:25

Last Modified:

09 Sep 2017 17:30

Publisher DOI:

10.1159/000353469

PubMed ID:

24335143

Uncontrolled Keywords:

Dental caries, Black stain, Dental plaque, Microbiota, Microorganisms

BORIS DOI:

10.7892/boris.47294

URI:

https://boris.unibe.ch/id/eprint/47294

Actions (login required)

Edit item Edit item
Provide Feedback