In situ fiber-optical monitoring of cytosolic calcium in tissue explant cultures

Ryser, Manuel; Künzi, Lisa; Geiser, Marianne; Frenz, Martin; Ricka, Jaro (2013). In situ fiber-optical monitoring of cytosolic calcium in tissue explant cultures. Journal of biophotonics, 8(3), pp. 183-195. Wiley-VCH 10.1002/jbio.201300089

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We present a fluorescence-lifetime based method for monitoring cell and tissue activity in situ, during cell culturing and in the presence of a strong autofluorescence background. The miniature fiber-optic probes are easily incorporated in the tight space of a cell culture chamber or in an endoscope. As a first application we monitored the cytosolic calcium levels in porcine tracheal explant cultures using the Calcium Green-5N (CG5N) indicator. Despite the simplicity of the optical setup we are able to detect changes of calcium concentration as small as 2.5 nM, with a monitoring time resolution of less than 1 s.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Institute of Applied Physics
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Cell Biology
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy

UniBE Contributor:

Ryser, Manuel; Künzi, Lisa; Geiser, Marianne; Frenz, Martin and Ricka, Jaroslav

Subjects:

500 Science > 530 Physics
500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1864-063X

Publisher:

Wiley-VCH

Language:

English

Submitter:

Marianne Geiser Kamber

Date Deposited:

02 May 2014 08:36

Last Modified:

18 Jan 2017 10:29

Publisher DOI:

10.1002/jbio.201300089

PubMed ID:

24026906

Uncontrolled Keywords:

Calcium Green-5N autofluorescence cell culture ciliated airway epithelium cytosolic calcium fiber-optic online monitoring ratiometric fluorescence spectroscopy

URI:

https://boris.unibe.ch/id/eprint/47763

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