Effects of ex vivo γ-tocopherol on airway macrophage function in healthy and mild allergic asthmatics

Geiser, Marianne; Lay, J. C.; Bennett, W. D.; Zhou, H.; Wang, X.; Peden, D. B.; Alexis, N. E. (2013). Effects of ex vivo γ-tocopherol on airway macrophage function in healthy and mild allergic asthmatics. Journal of innate immunity, 5(6), pp. 613-624. Karger 10.1159/000350234

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Elevated inflammation and altered immune responses are features found in atopic asthmatic airways. Recent studies indicate γ-tocopherol (GT) supplementation can suppress airway inflammation in allergic asthma. We studied the effects of in vitro GT supplementation on receptor-mediated phagocytosis and expression of cell surface molecules associated with innate and adaptive immunity on sputum-derived macrophages. Cells from nonsmoking healthy (n = 6) and mild house dust mite-sensitive allergic asthmatics (n = 6) were treated ex vivo with GT (300 µM) or saline (control). Phagocytosis of opsonized zymosan A bioparticles (Saccharomyces cerevisiae) and expression of surface molecules associated with innate and adaptive immunity were assessed using flow cytometry. GT caused significantly decreased (p < 0.05) internalization of attached zymosan bioparticles and decreased (p < 0.05) macrophage expression of CD206, CD36 and CD86 in allergic asthmatics but not in controls. Overall, GT caused downregulation of both innate and adaptive immune response elements, and atopic status appears to be an important factor.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Cell Biology
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy

UniBE Contributor:

Geiser, Marianne

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1662-8128

Publisher:

Karger

Language:

English

Submitter:

Marianne Geiser Kamber

Date Deposited:

02 May 2014 08:38

Last Modified:

18 Jan 2017 10:29

Publisher DOI:

10.1159/000350234

PubMed ID:

23689260

URI:

https://boris.unibe.ch/id/eprint/47777

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