Responses of lung cells to realistic exposure of primary and aged carbonaceous aerosols

Künzi, Lisa; Mertes, Peter; Schneider, Sarah; Jeannet, Natalie; Menzi, Carmela; Dommen, Josef; Baltensperger, Urs; Prévôt, André S.H.; Salathe, Matthias; Kalberer, Markus; Geiser, Marianne (2013). Responses of lung cells to realistic exposure of primary and aged carbonaceous aerosols. Atmospheric environment, 68, pp. 143-150. Pergamon 10.1016/j.atmosenv.2012.11.055

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Diesel exhaust and wood burning are important sources of ambient atmospheric particles due to increasing numbers of diesel cars and the importance of wood as a source of renewable energy. Inhalation is the predominant route of entry and uptake for fine and ultrafine particles into the body. Health effects of atmospheric particles are still not completely understood. There is consistent evidence from epidemiology that particle exposure contributes to respiratory and cardiovascular diseases.

This study aimed at examining acute responses of airway epithelial cells and luminal macrophages after exposure to freshly emitted and photochemically aged carbonaceous aerosols under realistic atmospheric conditions. In addition to a bronchial epithelial cell line advanced cell cultures namely fully differentiated respiratory epithelia and primary surface macrophages were used.

Our results demonstrate that a single exposure of the cells to realistic particle doses of 0.3–3 ng diesel or 3–9 ng wood aerosol per cm2 cell surface induces small, particle-specific responses. The release of interleukin-6 and -8 was found to be decreased in differentiated airway epithelia but not in the other cell models studied. Aerosol exposure decreased macrophage phagocytic activity by 45–90%. Cell and tissue integrity remained unaffected. Overall, primary and aged particles from the same combustion induced similar responses in the cell models tested, whereby particles from diesel exhaust affected the cells more than those from wood combustion.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Cell Biology 04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
UniBE Contributor:	Künzi, Lisa, Schaller, Sarah, Jeannet, Natalie, Menzi, Carmela-Priscilla, Geiser, Marianne
Subjects:	500 Science > 540 Chemistry 600 Technology > 610 Medicine & health
ISSN:	1352-2310
Publisher:	Pergamon
Language:	English
Submitter:	Marianne Geiser Kamber
Date Deposited:	02 May 2014 08:46
Last Modified:	02 Mar 2023 23:24
Publisher DOI:	10.1016/j.atmosenv.2012.11.055
URI:	https://boris.unibe.ch/id/eprint/47807