Nano-mechanical transduction of polymer micro-cantilevers to detect bio-molecular interactions

Urwyler, Prabitha; Köser, J.; Schift, Helmut; Gobrecht, Jens; Müller, Bert (2012). Nano-mechanical transduction of polymer micro-cantilevers to detect bio-molecular interactions. Biointerphases, 7(1-4), p. 6. American Vacuum Society 10.1007/s13758-011-0006-6

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Using variothermal polymer micro-injection molding, disposable arrays of eight polymer micro-cantilevers each 500 μm long, 100 μm wide and 25 μm thick were fabricated. The present study took advantage of an easy flow grade polypropylene. After gold coating for optical read-out and asymmetrical sensitization, the arrays were introduced into the Cantisens(®) Research system to perform mechanical and functional testing. We demonstrate that polypropylene cantilevers can be used as biosensors for medical purposes in the same manner as the established silicon ones to detect single-stranded DNA sequences and metal ions in real-time. A differential signal of 7 nm was detected for the hybridization of 1 μM complementary DNA sequences. For 100 nM copper ions the differential signal was found to be (36 ± 5) nm. Nano-mechanical sensing of medically relevant, nanometer-size species is essential for fast and efficient diagnosis.

Item Type:	Journal Article (Original Article)
Division/Institute:	10 Strategic Research Centers > ARTORG Center for Biomedical Engineering Research > ARTORG Center - Gerontechnology and Rehabilitation
UniBE Contributor:	Urwyler-Harischandra, Prabitha
Subjects:	500 Science > 570 Life sciences; biology 600 Technology > 610 Medicine & health 600 Technology > 620 Engineering
ISSN:	1934-8630
Publisher:	American Vacuum Society
Language:	English
Submitter:	Prabitha Urwyler-Harischandra
Date Deposited:	20 Jun 2014 11:09
Last Modified:	05 Dec 2022 14:32
Publisher DOI:	10.1007/s13758-011-0006-6
PubMed ID:	22589049
URI:	https://boris.unibe.ch/id/eprint/47918