Surface charge of polymer coated SPIONs influences the serum protein adsorption, colloidal stability and subsequent cell interaction in vitro

Hirsch, Vera; Kinnear, Calum; Moniatte, Marc; Rothen-Rutishauser, Barbara; Clift, Martin; Fink, Alke (2013). Surface charge of polymer coated SPIONs influences the serum protein adsorption, colloidal stability and subsequent cell interaction in vitro. Nanoscale, 5(9), pp. 3723-3732. Royal Society of Chemistry 10.1039/c2nr33134a

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It is known that the nanoparticle-cell interaction strongly depends on the physicochemical properties of the investigated particles. In addition, medium density and viscosity influence the colloidal behaviour of nanoparticles. Here, we show how nanoparticle-protein interactions are related to the particular physicochemical characteristics of the particles, such as their colloidal stability, and how this significantly influences the subsequent nanoparticle-cell interaction in vitro. Therefore, different surface charged superparamagnetic iron oxide nanoparticles were synthesized and characterized. Similar adsorbed protein profiles were identified following incubation in supplemented cell culture media, although cellular uptake varied significantly between the different particles. However, positively charged nanoparticles displayed a significantly lower colloidal stability than neutral and negatively charged particles while showing higher non-sedimentation driven cell-internalization in vitro without any significant cytotoxic effects. The results of this study strongly indicate therefore that an understanding of the aggregation state of NPs in biological fluids is crucial in regards to their biological interaction(s).

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Pneumologie (Erwachsene)
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology

UniBE Contributor:

Rothen-Rutishauser, Barbara and Clift, Martin

Subjects:

600 Technology > 610 Medicine & health

ISSN:

2040-3364

Publisher:

Royal Society of Chemistry

Language:

English

Submitter:

Rahel Holderegger

Date Deposited:

30 Apr 2014 10:14

Last Modified:

22 Dec 2015 10:20

Publisher DOI:

10.1039/c2nr33134a

PubMed ID:

23334060

URI:

https://boris.unibe.ch/id/eprint/48219

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