Consistency of thromboelastometry analysis under scrutiny: Results of a systematic evaluation within and between analysers.

Nagler, Michael; Ten Cate, H; Kathriner, S; Casutt, M; Bachmann, L M; Wuillemin, W A (2014). Consistency of thromboelastometry analysis under scrutiny: Results of a systematic evaluation within and between analysers. Thrombosis and haemostasis, 111(6), pp. 1161-1166. Schattauer 10.1160/TH13-10-0870

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While the use of thromboelastometry analysis (ROTEM®) in evaluation of haemostasis is rapidly increasing, important validity parameters of testing remain inadequately examined. We aimed to study systematically the consistency of thromboelastometry parameters within individual tests regarding measurements between different analysers, between different channels of the same analyser, between morning and afternoon measurements (circadian variation), and if measured four weeks apart. Citrated whole blood samples from 40 healthy volunteers were analysed with two analysers in parallel. EXTEM, INTEM, FIBTEM, HEPTEM and APTEM tests were conducted. A Bland-Altman comparison was performed and homogeneity of variances was tested using the pitman test. P-value ranges were used to classify the level of homogeneity (p<0.15 - low homogeneity, p = 0.15 to 0.5 - intermediate homogeneity, p>0.5 high homogeneity). Less than half of all comparisons made showed high homogeneity of variances (p>0.5) and in about a fifth of comparisons data distributions were heterogeneous (p<0.15). There was no clear pattern for homogeneity. On average, comparisons of MCF, ML and LI30 measurements tended to be better, but none of the tests assessed outperformed another. In conclusion, systematic investigation reveals large differences in the results of some thromboelastometry parameters and lack of consistency. Clinicians and scientists should take these inconsistencies into account and focus on parameters with a higher homogeneity such as MCF.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Haematology and Central Haematological Laboratory

UniBE Contributor:

Nagler, Michael

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0340-6245

Publisher:

Schattauer

Submitter:

Verena Zwahlen

Date Deposited:

23 Jul 2014 09:53

Last Modified:

20 Dec 2014 13:51

Publisher DOI:

10.1160/TH13-10-0870

PubMed ID:

24477424

Uncontrolled Keywords:

Thromboelastometry reliability reproducibility

BORIS DOI:

10.7892/boris.48335

URI:

https://boris.unibe.ch/id/eprint/48335

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