Infected pancreatic necrosis increases the severity of experimental necrotizing pancreatitis in mice

Poxleitner, Philipp J.; Seifert, Gabriel; Richter, Sabine; Hopt, Ulrich T.; Wittel, Uwe A. (2013). Infected pancreatic necrosis increases the severity of experimental necrotizing pancreatitis in mice. Pancreas, 42(7), pp. 1150-1156. Lippincott Williams & Wilkins 10.1097/MPA.0b013e318291c251

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OBJECTIVE Infection of pancreatic necrosis in necrotizing pancreatitis increases the lethality of patients with acute pancreatitis. To examine mechanisms underlying this clinical observation, we developed and tested a model, in which primary infection of necrosis is achieved in taurocholate-induced pancreatitis in mice. METHODS Sterile necrosis of acute necrotizing pancreatitis was induced by retrograde injection of 4% taurocholate into the common bile duct of Balb/c mice. Primary infection of pancreatic necrosis was induced by coinjecting 10 colony-forming units of Escherichia coli. Animals were killed after 6, 12, 24, 48, and 120 hours, and pancreatic damage and pancreatitis-associated systemic inflammatory response were assessed. RESULTS Mice with pancreatic acinar cell necrosis had an increased bacterial concentration in all tissues and showed sustained bacteremia. Acute pancreatitis was induced only by coinjection of taurocholate and not by bacterial infection alone. Infection of pancreatic necrosis increased pancreatic damage and the pulmonary vascular leak. Serum glucose concentrations serving as a parameter of hepatic function were reduced in mice with infected pancreatic necrosis. CONCLUSIONS Primary infection of pancreatic necrosis with E. coli increases both pancreatic damage and pulmonary and hepatic complications in acute necrotizing pancreatitis in mice.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Medical Education > Institute for Medical Education

UniBE Contributor:

Richter, Cornelia Sabine

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0885-3177

Publisher:

Lippincott Williams & Wilkins

Language:

English

Submitter:

Eveline Götschmann-Meile

Date Deposited:

01 Apr 2014 12:09

Last Modified:

16 Jan 2017 14:58

Publisher DOI:

10.1097/MPA.0b013e318291c251

PubMed ID:

24048455

URI:

https://boris.unibe.ch/id/eprint/48557

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