Increased levels of biglycan in endometriomas and peritoneal fluid samples from ovarian endometriosis patients

Kocbek, Vida; Hevir-Kene, Neli; Bersinger, Nick A.; Mueller, Michael; Rižner, Tea Lanišnik (2014). Increased levels of biglycan in endometriomas and peritoneal fluid samples from ovarian endometriosis patients. Gynecological endocrinology, 30(7), pp. 520-524. Informa Healthcare 10.3109/09513590.2014.898055

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Abstract In our previous low-density-array gene-expression analysis we found an increased expression of biglycan gene in ovarian endometriosis patients. In the present study we evaluated biglycan expression at the protein level in tissue, serum and peritoneal fluid (PF) from ovarian endometriosis patients, patients with benign ovarian cysts and healthy women. Twenty samples of endometriomas and 27 of control tissues (benign ovarian cysts and eutopic endometrium of healthy women) were obtained laparoscopically or by curettage. Serum and PF samples were collected from 56 ovarian endometriosis patients and 40 controls (patients with benign cysts and healthy women). Tissue biglycan levels and serum and PF biglycan concentrations were determined by Western blotting and ELISA, respectively. Biglycan was detected in endometriomas and in benign cysts tissues but differed in glycosylation levels. The PF biglycan concentrations were significantly increased in ovarian endometriosis patients (mean ± SD = 220.3 ± 190.5 pg/mg protein) compared to the whole control group (101.9 ± 94.7 pg/mg protein, p < 0.001), while serum concentrations did not differ significantly. Biglycan appears to be involved in ovarian pathologies and probably has different roles in benign cysts as compared to ovarian endometriomas.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Endometriose und gynäkologische Onkologie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Endometriose und gynäkologische Onkologie

04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Gynaecology

UniBE Contributor:

Kocbek, Vida; Bersinger, Nick A. and Mueller, Michael

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0951-3590

Publisher:

Informa Healthcare

Language:

English

Submitter:

Monika Zehr

Date Deposited:

17 May 2016 11:23

Last Modified:

17 May 2016 11:23

Publisher DOI:

10.3109/09513590.2014.898055

PubMed ID:

24641675

URI:

https://boris.unibe.ch/id/eprint/48963

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