β1 integrins regulate myoblast fusion and sarcomere assembly

Schwander, Martin; Leu, Marco; Stumm, Michael; Dorchies, Olivier M.; Ruegg, Urs T.; Schittny, Johannes; Müller, Ulrich (2003). β1 integrins regulate myoblast fusion and sarcomere assembly. Developmental cell, 4(5), pp. 673-685. Cell Press 10.1016/S1534-5807(03)00118-7

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The mechanisms that regulate the formation of multinucleated muscle fibers from mononucleated myoblasts are not well understood. We show here that extracellular matrix (ECM) receptors of the beta1 integrin family regulate myoblast fusion. beta1-deficient myoblasts adhere to each other, but plasma membrane breakdown is defective. The integrin-associated tetraspanin CD9 that regulates cell fusion is no longer expressed at the cell surface of beta1-deficient myoblasts, suggesting that beta1 integrins regulate the formation of a protein complex important for fusion. Subsequent to fusion, beta1 integrins are required for the assembly of sarcomeres. Other ECM receptors such as the dystrophin glycoprotein complex are still expressed but cannot compensate for the loss of beta1 integrins, providing evidence that different ECM receptors have nonredundant functions in skeletal muscle fibers.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Functional Anatomy

UniBE Contributor:

Schittny, Johannes

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

1534-5807

Publisher:

Cell Press

Language:

English

Submitter:

Johannes Schittny

Date Deposited:

01 Sep 2014 11:02

Last Modified:

06 Dec 2014 21:18

Publisher DOI:

10.1016/S1534-5807(03)00118-7

PubMed ID:

12737803

BORIS DOI:

10.7892/boris.50153

URI:

https://boris.unibe.ch/id/eprint/50153

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