Use of Genetically Modified Glial Cells Overexpressing Laminin α1-Chain Peptides in Neurite Outgrowth Studies

Webersinke, Gerald; Bauer, Hans-Chr.; Danninger, Claudia; Krizbai, Istvan A.; Schittny, Johannes C.; Thalhamer, Josef; Bauer, Hannelore (2000). Use of Genetically Modified Glial Cells Overexpressing Laminin α1-Chain Peptides in Neurite Outgrowth Studies. Cellular and molecular neurobiology, 20(6), pp. 605-622. Springer 10.1023/A:1007004706028

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1. C6 glioma cells were transfected with two constructs carrying C-terminal laminin alpha1-chain sequences of 117 and 114 bp length, respectively. These sequences are specifically known to code for peptides which have neurite-promoting activity. 2. The stable expression and secretion of the two peptides was detected by Northern and Western blot analysis. 3. Primary neuronal cultures derived from embryonic mouse forebrain were cocultured with these transfected cells and exhibited a substantial increase in neurite outgrowth and in survival time. Conditioned media from the transfected cells generated similar effects. 4. Organotypic cultures from embryonic mouse brain were used as a second system as being closer to the in vivo situation. Again, coculture of brain slices with transfected cells or treatment with laminin peptide-containing media increased neuronal outgrowth.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Functional Anatomy

UniBE Contributor:

Schittny, Johannes

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

0272-4340

Publisher:

Springer

Submitter:

Johannes Schittny

Date Deposited:

25 Aug 2014 14:06

Last Modified:

28 Dec 2014 16:50

Publisher DOI:

10.1023/A:1007004706028

PubMed ID:

11100971

Uncontrolled Keywords:

Neurite outgrowth, Laminin peptides, Transfection, Glia

BORIS DOI:

10.7892/boris.50157

URI:

https://boris.unibe.ch/id/eprint/50157

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