The beta-isoform of neuronal nitric oxide synthase (nNOS) lacking the PDZ domain is localized at the sarcolemma

Baum, Oliver; Schläppi, Simon; Huber-Abel, Felicitas A; Weichert, Alexander; Hoppeler, Hans; Zakrzewicz, Andreas (2011). The beta-isoform of neuronal nitric oxide synthase (nNOS) lacking the PDZ domain is localized at the sarcolemma. FEBS letters, 585(20), pp. 3219-23. Amsterdam: Elsevier 10.1016/j.febslet.2011.09.016

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In skeletal muscles, the expression of neuronal NO synthase (nNOS) isoforms is uncharacterized at the protein level. We therefore conducted epitope mapping with anti-peptide-antibodies. Antibodies specific for the nNOS N-terminus recognized the 160-kDa alpha-isoform. In contrast, antibodies against the middle portion or the C-terminus of nNOS bound additionally to the truncated 140-kDa beta-isoform which lacks the PDZ-domain present in the alpha-isoform. All nNOS immunohistochemical reactivity was confined to the sarcolemma. Consistently, immunoblotting disclosed both nNOS-isoforms to be co-enriched in the membrane-associated fractions. The beta-isoform was co-immunoprecipitated with alpha-isoform antibodies in muscle extracts indicating an association of both nNOS-isoforms to direct the beta-variant to the sarcolemma.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Functional Anatomy
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy

UniBE Contributor:

Baum, Oliver and Hoppeler, Hans-Heinrich

ISSN:

0014-5793

Publisher:

Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:17

Last Modified:

17 Mar 2015 19:22

Publisher DOI:

10.1016/j.febslet.2011.09.016

PubMed ID:

21945318

Web of Science ID:

000296514100011

URI:

https://boris.unibe.ch/id/eprint/5114 (FactScience: 209832)

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