The adhesion modulating properties of tenascin-w

Brellier, Florence; Martina, Enrico; Chiquet, Matthias; Ferralli, Jacqueline; van der Heyden, Michael; Orend, Gertraud; Schittny, Johannes C; Chiquet-Ehrismann, Ruth; Tucker, Richard P (2012). The adhesion modulating properties of tenascin-w. International journal of biological sciences, 8(2), pp. 187-94. Lake Haven (Aus.): Ivyspring International 10.7150/ijbs.8.187

Full text not available from this repository.

Tenascins are extracellular matrix glycoproteins associated with cell motility, proliferation and differentiation. Tenascin-C inhibits cell spreading by binding to fibronectin; tenascin-R and tenascin-X also have anti-adhesive properties in vitro. Here we have studied the adhesion modulating properties of the most recently characterized tenascin, tenascin-W. C2C12 cells, a murine myoblast cell line, will form broad lamellipodia with stress fibers and focal adhesion complexes after culture on fibronectin. In contrast, C2C12 cells cultured on tenascin-W fail to spread and form stress fibers or focal adhesion complexes, and instead acquire a multipolar shape with short, actin-tipped pseudopodia. The same stellate morphology is observed when C2C12 cells are cultured on a mixture of fibronectin and tenascin-W, or on fibronectin in the presence of soluble tenascin-W. Tenascin-W combined with fibronectin also inhibits the spreading of mouse embryo fibroblasts when compared with cells cultured on fibronectin alone. The similarity between the adhesion modulating effects of tenascin-W and tenascin-C in vitro led us to study the possibility of tenascin-W compensating for tenascin-C in tenascin-C knockout mice, especially during epidermal wound healing. Dermal fibroblasts harvested from a tenascin-C knockout mouse express tenascin-W, but dermal fibroblasts taken from a wild type mouse do not. However, there is no upregulation of tenascin-W in the dermis of tenascin-C knockout mice, or in the granulation tissue of skin wounds in tenascin-C knockout animals. Similarly, tenascin-X is not upregulated in early wound granulation tissue in the tenascin-C knockout mice. Thus, tenascin-W is able to inhibit cell spreading in vitro and it is upregulated in dermal fibroblasts taken from the tenascin-C knockout mouse, but neither it nor tenascin-X are likely to compensate for missing tenascin-C during wound healing.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > School of Dental Medicine > Department of Orthodontics 04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Functional Anatomy
UniBE Contributor:	Chiquet, Matthias, Schittny, Johannes
ISSN:	1449-2288
Publisher:	Ivyspring International
Language:	English
Submitter:	Eveline Carmen Schuler
Date Deposited:	04 Oct 2013 14:17
Last Modified:	05 Dec 2022 14:04
Publisher DOI:	10.7150/ijbs.8.187
PubMed ID:	22211116
Web of Science ID:	000301061100003
URI:	https://boris.unibe.ch/id/eprint/5120 (FactScience: 209838)