Subgingival microflora in inflammatory bowel disease patients with untreated periodontitis

Brito, Fernanda; Zaltman, Cyrla; Carvalho, Ana T. P.; Fischer, Ricardo G.; Persson, Rutger; Gustafsson, Anders; Figueredo, Carlos M. S. (2013). Subgingival microflora in inflammatory bowel disease patients with untreated periodontitis. European journal of gastroenterology & hepatology, 25(2), pp. 239-245. Lippincott Williams & Wilkins 10.1097/MEG.0b013e32835a2b70

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OBJECTIVE To analyze the subgingival microflora composition of inflammatory bowel disease (IBD) patients with untreated chronic periodontitis and compare them with systemically healthy controls also having untreated chronic periodontitis. METHOD Thirty IBD patients [15 with Crohn's disease (CD) and 15 with ulcerative colitis (UC)] and 15 control individuals participated in the study. All patients had been diagnosed with untreated chronic periodontitis. From every patient, subgingival plaque was collected from four gingivitis and four periodontitis sites with paper points. Samples from the same category (gingivitis or periodontitis) in each patient were pooled together and stored at -70 °C until analysis using a checkerboard DNA-DNA hybridization technique for 74 bacterial species. RESULTS Multiple-comparison analysis showed that the groups differed in bacterial counts for Bacteroides ureolyticus, Campylobacter gracilis, Parvimonas micra, Prevotella melaninogenica, Peptostreptococcus anaerobius, Staphylococcus aureus, Streptococcus anginosus, Streptococcus intermedius, Streptococcus mitis, Streptococcus mutans, and Treponema denticola (P<0.001). CD patients had significantly higher levels of these bacteria than UC patients either in gingivitis or in periodontitis sites (P<0.05). CD patients harbored higher levels of P. melaninogenica, S. aureus, S. anginosus, and S. mutans compared with controls both at gingivitis and at periodontitis sites (P<0.05). UC patients harbored higher levels of S. aureus (P=0.01) and P. anaerobius (P=0.05) than controls only in gingivitis sites. CONCLUSION Our study showed that even with similar clinical periodontal parameters, IBD patients harbor higher levels of bacteria that are related to opportunistic infections in inflamed subgingival sites that might be harmful for the crucial microbe-host interaction.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology

UniBE Contributor:

Persson, Gösta Rutger

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0954-691X

Publisher:

Lippincott Williams & Wilkins

Language:

English

Submitter:

Eveline Carmen Schuler

Date Deposited:

01 May 2014 10:48

Last Modified:

28 Sep 2017 18:53

Publisher DOI:

10.1097/MEG.0b013e32835a2b70

PubMed ID:

23060013

BORIS DOI:

10.7892/boris.51680

URI:

https://boris.unibe.ch/id/eprint/51680

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