Clathrin heavy chain plays multiple roles in polarizing the Drosophila oocyte downstream of Bic-D

Vazquez Pianzola, Maria Paula; Adam, Jacqueline; Haldemann, Dominique; Hain, Daniel; Suter, Beat; Urlaub, Henning (2014). Clathrin heavy chain plays multiple roles in polarizing the Drosophila oocyte downstream of Bic-D. Development, 141(9), pp. 1915-1926. Company of Biologists Limited 10.1242/dev.099432

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Bicaudal-D (Bic-D), Egalitarian (Egl), microtubules and their motors
form a transport machinery that localizes a remarkable diversity
of mRNAs to specific cellular regions during oogenesis and
embryogenesis. Bic-D family proteins also promote dynein-dependent
transport of Golgi vesicles, lipid droplets, synaptic vesicles and nuclei.
However, the transport of these different cargoes is still poorly
understood. We searched for novel proteins that either mediate Bic-Ddependent
transport processes or are transported by them. Clathrin
heavy chain (Chc) co-immunopurifies with Bic-D in embryos and
ovaries, and a fraction of Chc colocalizes with Bic-D. Both proteins
control posterior patterning of the Drosophila oocyte and endocytosis.
Although the role of Chc in endocytosis is well established, our results
show that Bic-D is also needed for the elevated endocytic activity at the
posterior of the oocyte. Apart fromaffecting endocytosis indirectly by its
role in osk mRNA localization, Bic-D is also required to transport Chc
mRNA into the oocyte and for transport and proper localization of Chc
protein to the oocyte cortex, pointing to an additional,more direct role of
Bic-D in the endocytic pathway. Furthermore, similar to Bic-D, Chc also
contributes to proper localization of osk mRNA and to oocyte growth.
However, in contrast to other endocytic components and factors of the
endocytic recycling pathway, such as Rabenosyn-5 (Rbsn-5) and
Rab11, Chc is needed during early stages of oogenesis (from stage 6
onwards) to localize oskmRNA correctly.Moreover,we also uncovered
a novel, presumably endocytosis-independent, role of Chc in the
establishment of microtubule polarity in stage 6 oocytes.

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