Clathrin heavy chain plays multiple roles in polarizing the Drosophila oocyte downstream of Bic-D

Vazquez Pianzola, Maria Paula; Adam, Jacqueline; Haldemann, Dominique; Hain, Daniel; Suter, Beat; Urlaub, Henning (2014). Clathrin heavy chain plays multiple roles in polarizing the Drosophila oocyte downstream of Bic-D. Development, 141(9), pp. 1915-1926. Company of Biologists Limited 10.1242/dev.099432

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Bicaudal-D (Bic-D), Egalitarian (Egl), microtubules and their motors form a transport machinery that localizes a remarkable diversity of mRNAs to specific cellular regions during oogenesis and embryogenesis. Bic-D family proteins also promote dynein-dependent transport of Golgi vesicles, lipid droplets, synaptic vesicles and nuclei. However, the transport of these different cargoes is still poorly understood. We searched for novel proteins that either mediate Bic-Ddependent transport processes or are transported by them. Clathrin heavy chain (Chc) co-immunopurifies with Bic-D in embryos and ovaries, and a fraction of Chc colocalizes with Bic-D. Both proteins control posterior patterning of the Drosophila oocyte and endocytosis. Although the role of Chc in endocytosis is well established, our results show that Bic-D is also needed for the elevated endocytic activity at the posterior of the oocyte. Apart fromaffecting endocytosis indirectly by its role in osk mRNA localization, Bic-D is also required to transport Chc mRNA into the oocyte and for transport and proper localization of Chc protein to the oocyte cortex, pointing to an additional,more direct role of Bic-D in the endocytic pathway. Furthermore, similar to Bic-D, Chc also contributes to proper localization of osk mRNA and to oocyte growth. However, in contrast to other endocytic components and factors of the endocytic recycling pathway, such as Rabenosyn-5 (Rbsn-5) and Rab11, Chc is needed during early stages of oogenesis (from stage 6 onwards) to localize oskmRNA correctly.Moreover,we also uncovered a novel, presumably endocytosis-independent, role of Chc in the establishment of microtubule polarity in stage 6 oocytes.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology
08 Faculty of Science > Department of Biology > Institute of Cell Biology > Drosophila

UniBE Contributor:

Vazquez Pianzola, Maria Paula; Adam, Jacqueline; Haldemann, Dominique; Hain, Daniel and Suter, Beat

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

0950-1991

Publisher:

Company of Biologists Limited

Language:

English

Submitter:

Jacqueline Schmuckli

Date Deposited:

27 Apr 2014 15:31

Last Modified:

26 Jun 2016 01:52

Publisher DOI:

10.1242/dev.099432

BORIS DOI:

10.7892/boris.51681

URI:

https://boris.unibe.ch/id/eprint/51681

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