Sequence of the 50-kb conjugative multiresistance plasmid pRE25 from Enterococcus faecalis RE25

Schwarz, Franziska V.; Perreten, Vincent; Teuber, Michael (2001). Sequence of the 50-kb conjugative multiresistance plasmid pRE25 from Enterococcus faecalis RE25. Plasmid, 46(3), pp. 170-187. Elsevier 10.1006/plas.2001.1544

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The complete 50,237-bp DNA sequence of the conjugative and mobilizing multiresistance plasmid pRE25 from Enterococcus faecalis RE25 was determined. The plasmid had 58 putative open reading frames, 5 of which encode resistance to 12 antimicrobials. Chloramphenicol acetyltransferase and the 23S RNA methylase are identical to gene products of the broad-host-range plasmid pIP501 from Streptococcus agalactiae. In addition, a 30.5-kb segment is almost identical to pIP501. Genes encoding an aminoglycoside 6-adenylyltransferase, a streptothricin acetyltransferase, and an aminoglycoside phosphotransferase are arranged in tandem on a 7.4-kb fragment as previously reported in Tn5405 from Staphylococcus aureus and in pJH1 from E. faecalis. One interrupted and five complete IS elements as well as three replication genes were also identified. pRE25 was transferred by conjugation to E. faecalis, Listeria innocua, and Lactococcus lactis by means of a transfer region that appears similar to that of pIP501. It is concluded that pRE25 may contribute to the further spread of antibiotic-resistant microorganisms via food into the human community.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Perreten, Vincent

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture

ISSN:

0147-619X

Publisher:

Elsevier

Language:

English

Submitter:

Vincent Perreten

Date Deposited:

01 Sep 2014 09:17

Last Modified:

01 Sep 2014 09:17

Publisher DOI:

10.1006/plas.2001.1544

PubMed ID:

11735367

Uncontrolled Keywords:

antibiotic resistance, Enterococcus, food, multiresistance, plasmid, conjugation, transfer

URI:

https://boris.unibe.ch/id/eprint/52026

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