Antibiotic resistance genes in coagulase-negative staphylococci isolated from food

Perreten, Vincent; Giampà, Natalina; Schuler-Schmid, Ursula; Teuber, Michael (1998). Antibiotic resistance genes in coagulase-negative staphylococci isolated from food. Systematic and applied microbiology, 21(1), pp. 113-120. Elsevier 10.1016/S0723-2020(98)80014-3

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Coagulase-negative staphylococci were isolated from different raw milk cheeses and raw meat products and screened for their antibiotic resistances. They were identified as Staphylococcus xylosus, S. lentus, S. caprae, S. epidemidis and S. haemolyticus. The most frequent resistances found were those to chloramphenicol, tetracycline, erythromycin and lincomycin. They have been characterized on the molecular level. The chloramphenicol resistance genes were localized in several S. xylosus and S. caprae on plasmids with sizes ranging from 3.8-kb to 4.3-kb and were identified as chloramphenicol acetyltransferase (cat). All the tetracycline resistant strains were identified as S. xylosus and harboured a 4.4-kb plasmid carrying the tetracycline efflux resistance gene (tetK). The two erythromycin/lincomycin resistant S. caprae and S. epidermidis strains did not hybridize with the MLSB resistance genes ermAM, ermA, ermB and ermC. Three erythromycin resistant Staphylococcus sp. strains harboured an erythromycin efflux resistance gene (msr) localized twice on a 18-kb plasmid and once on the chromosome. A S. haemolyticus strain showing resistance to both lincomycin and clindamycin harboured a linA gene-carrying 2.2-kb plasmid. Further resistances to gentamicin, penicillin and kanamycin were less frequently observed and yet not characterized on a molecular level.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Perreten, Vincent

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology

ISSN:

0723-2020

Publisher:

Elsevier

Language:

English

Submitter:

Vincent Perreten

Date Deposited:

22 Sep 2014 09:12

Last Modified:

22 Sep 2014 09:12

Publisher DOI:

10.1016/S0723-2020(98)80014-3

PubMed ID:

9741115

URI:

https://boris.unibe.ch/id/eprint/52029

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