Capper, C.; Kaelin-Lang, A. (2013). Locomotor velocity and striatal adaptive gene expression changes of the direct and indirect pathways in Parkinsonian rats. Journal of Parkinson's disease, 3(3), pp. 341-349. IOS Press 10.3233/JPD-130202
Full text not available from this repository. (Request a copy)BACKGROUND
In Parkinson's disease (PD), bradykinesia, or slowness of movement, only appears after a large striatal dopamine depletion. Compensatory mechanisms probably play a role in this delayed appearance of symptoms.
OBJECTIVE
Our hypothesis is that the striatal direct and indirect pathways participate in these compensatory mechanisms.
METHODS
We used the unilateral 6-hydroxydopamine (6-OHDA) rat model of PD and control animals. Four weeks after the lesion, the spontaneous locomotor activity of the rats was measured and then the animals were killed and their brain extracted. We quantified the mRNA expression of markers of the striatal direct and indirect pathways as well as the nigral expression of dopamine transporter (DAT) and tyrosine hydroxylase (TH) mRNA. We also carried out an immunohistochemistry for the striatal TH protein expression.
RESULTS
As expected, the unilateral 6-OHDA rats presented a tendency to an ipsilateral head turning and a low locomotor velocity. In 6-OHDA rats only, we observed a significant and positive correlation between locomotor velocity and both D1-class dopamine receptor (D1R) (direct pathway) and enkephalin (ENK) (indirect pathway) mRNA in the lesioned striatum, as well as between D1R and ENK mRNA.
CONCLUSIONS
Our results demonstrate a strong relationship between both direct and indirect pathways and spontaneous locomotor activity in the parkinsonian rat model. We suggest a synergy between both pathways which could play a role in compensatory mechanisms and may contribute to the delayed appearance of bradykinesia in PD.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Neurology |
UniBE Contributor: |
Capper, Christine, Kaelin, Alain |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1877-718X |
Publisher: |
IOS Press |
Language: |
English |
Submitter: |
Ursula Aecherli Tobler |
Date Deposited: |
04 Jun 2014 14:27 |
Last Modified: |
05 Dec 2022 14:34 |
Publisher DOI: |
10.3233/JPD-130202 |
PubMed ID: |
23948996 |
URI: |
https://boris.unibe.ch/id/eprint/52110 |
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