Identification and quantification of phytochelatins in roots of rice to long-term exposure: evidence of individual role on arsenic accumulation and translocation

Lemos Batista, Bruno; Nigar, Meher; Mestrot, Adrien; Alves Rocha, Bruno; Barbosa Junior, Fernando; Price, Adam H.; Raab, Andrea; Feldmann, Jörg (2014). Identification and quantification of phytochelatins in roots of rice to long-term exposure: evidence of individual role on arsenic accumulation and translocation. Journal of Experimental Botany, 65(6), pp. 1467-1479. Oxford University Press 10.1093/jxb/eru018

[img]
Preview
Text
10.1093-jxb-eru018.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (2MB) | Preview
[img]
Preview
Text
eru018.pdf - Other
Available under License ["licenses_description_cc-by" not defined].

Download (2MB) | Preview

Rice has the predilection to take up arsenic in the form of methylated arsenic (o-As) and inorganic arsenic species (i-As). Plants defend themselves using i-As efflux systems and the production of phytochelatins (PCs) to complex i-As. Our study focused on the identification and quantification of phytochelatins by HPLC-ICP-MS/ESI-MS, relating them to the several variables linked to As exposure. GSH, 11 PCs, and As–PC complexes from the roots of six rice cultivars (Italica Carolina, Dom Sofid, 9524, Kitrana 508, YRL-1, and Lemont) exposed to low and high levels of i-As were compared with total, i-As, and o-As in roots, shoots, and grains. Only Dom Sofid, Kitrana 508, and 9524 were found to produce higher levels of PCs even when exposed to low levels of As. PCs were only correlated to i-As in the roots (r=0.884, P <0.001). However, significant negative correlations to As transfer factors (TF) roots–grains (r= –0.739, P <0.05) and shoots–grains (r= –0.541, P <0.05), suggested that these peptides help in trapping i-As but not o-As in the roots, reducing grains’ i-As. Italica Carolina reduced i-As in grains after high exposure, where some specific PCs had a special role in this reduction. In Lemont, exposure to elevated levels of i-As did not result in higher i-As levels in the grains and there were no significant increases in PCs or thiols. Finally, the high production of PCs in Kitrana 508 and Dom Sofid in response to high As treatment did not relate to a reduction of i-As in grains, suggesting that other mechanisms such as As–PC release and transport seems to be important in determining grain As in these cultivars.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Institute of Geography > Physical Geography > Unit Soil Science
08 Faculty of Science > Institute of Geography

UniBE Contributor:

Mestrot, Adrien

Subjects:

900 History > 910 Geography & travel

ISSN:

0022-0957

Publisher:

Oxford University Press

Language:

English

Submitter:

Monika Wälti-Stampfli

Date Deposited:

15 Sep 2014 14:12

Last Modified:

27 Apr 2018 10:24

Publisher DOI:

10.1093/jxb/eru018

BORIS DOI:

10.7892/boris.52375

URI:

https://boris.unibe.ch/id/eprint/52375

Actions (login required)

Edit item Edit item
Provide Feedback