A pyrosequencing assay for the rapid discrimination of mitochondrial lineages in the Salmo trutta species complex

Keller, I.; Taverna, A.; Seehausen, Ole (2011). A pyrosequencing assay for the rapid discrimination of mitochondrial lineages in the Salmo trutta species complex. Molecular ecology resources, 11(1), pp. 196-199. Oxford: Blackwell Science 10.1111/j.1755-0998.2010.02897.x

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The European trout (Salmo trutta species complex) is genetically very diverse consisting of five distinct mitochondrial lineages that probably originated in the Pleistocene. Here, we describe a novel pyrosequencing protocol to generate two short sequence reads from the mitochondrial control region, which allow the unambiguous identification of all five lineages. The approach was found to be easily transferable between laboratories and should be a valuable tool for the assessment of genetic diversity in trout. Pyrosequencing-based assays for molecular species identification are expected to be generally useful whenever multiple positions in a short DNA sequence need to be assessed.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Ecology and Evolution (IEE) > Aquatic Ecology

UniBE Contributor:

Seehausen, Ole

ISSN:

1755-098X

ISBN:

1755-098X

Publisher:

Blackwell Science

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:17

Last Modified:

06 Dec 2013 13:25

Publisher DOI:

10.1111/j.1755-0998.2010.02897.x

Web of Science ID:

000285249800028

URI:

https://boris.unibe.ch/id/eprint/5253 (FactScience: 209984)

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