Cerebrospinal-fluid cytokine and chemokine profile in patients with pneumococcal and meningococcal meningitis

Coutinho, Leonam G; Grandgirard, Denis; Leib, Stephen L.; Agnez-Lima, Lucymara F (2013). Cerebrospinal-fluid cytokine and chemokine profile in patients with pneumococcal and meningococcal meningitis. BMC infectious diseases, 13(1), p. 326. BioMed Central 10.1186/1471-2334-13-326

[img]
Preview
Text
1471-2334-13-326.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (798kB) | Preview

BACKGROUND Bacterial meningitis is characterized by an intense inflammatory reaction contributing to neuronal damage. The aim of this study was to obtain a comparative analysis of cytokines and chemokines in patients with pneumococcal (PM) and meningococcal meningitis (MM) considering that a clear difference between the immune response induced by these pathogens remains unclear. METHODS The cyto/chemokines, IL-1beta, IL-2, IL-6, TNF-alpha, IFN-gamma, IL-10, IL-1Ra, CXCL8/IL-8, CCL2/MCP-1, CLL3/MIP-1alpha, CCL4/MIP-1gamma and G-CSF, were measured in cerebrospinal fluid (CSF) samples from patients with PM and MM. Additionally, a literature review about the expression of cytokines in CSF samples of patients with MB was made. RESULTS Concerning cytokines levels, only IFN-gamma was significantly higher in patients with Streptococcus pneumoniae compared to those with Neisseria meningitidis, regardless of the time when the lumbar puncture (LP) was made. Furthermore, when samples were compared considering the timing of the LP, higher levels of TNF-alpha (P <0.05) were observed in MM patients whose LP was made within 48 h from the initial symptoms of disease. We also observed that the index of release of cyto/chemokines per cell was significantly higher in PM. From the literature review, it was observed that TNF-alpha, IL-1beta and IL-6 are the best studied cytokines, while reports describing the concentration of the cytokine IL-2, IL-1Ra, G-CSF and CCL4/MIP-1beta in CSF samples of patients with bacterial meningitis were not found. CONCLUSION The data obtained in this study and the previously published data show a similar profile of cytokine expression during PM and MM. Nevertheless, the high levels of IFN-gamma and the ability to release high levels of cytokines with a low number of cells are important factors to be considered in the pathogenesis of PM and thereby should be further investigated. Moreover, differences in the early response induced by the pathogens were observed. However, the differences observed are not sufficient to trigger changes in the current therapy of corticosteroids adopted in both the PM and MM.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases

UniBE Contributor:

Grandgirard, Denis and Leib, Stephen

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1471-2334

Publisher:

BioMed Central

Funders:

[UNSPECIFIED] UBS Optimus Foundation

Language:

English

Submitter:

Stephen Leib

Date Deposited:

26 May 2014 09:08

Last Modified:

08 Feb 2017 13:49

Publisher DOI:

10.1186/1471-2334-13-326

PubMed ID:

23865742

Uncontrolled Keywords:

Pneumococcal meningitis, Meningococcal meningitis, Cytokines, Chemokines, Interferon gamma, Cerebrospinal fluid

BORIS DOI:

10.7892/boris.52655

URI:

https://boris.unibe.ch/id/eprint/52655

Actions (login required)

Edit item Edit item
Provide Feedback