Impaired cortical energy metabolism but not major antioxidant defenses in experimental bacterial meningitis.

Ghielmetti, Marco; Ren, Hao; Leib, Stephen; Täuber, Martin G.; Christen, Stephan (2003). Impaired cortical energy metabolism but not major antioxidant defenses in experimental bacterial meningitis. Brain research, 976(2), pp. 139-148. Elsevier 10.1016/S0006-8993(03)02557-5

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The loss of soluble brain antioxidants and protective effects of radical scavengers implicate reactive oxygen species in cortical neuronal injury caused by bacterial meningitis. However, the lack of significant oxidative damage in cortex [J. Neuropathol. Exp. Neurol. 61 (2002) 605-613] suggests that cortical neuronal injury may not be due to excessive parenchymal oxidant production. To see whether this tissue region exhibits a prooxidant state in bacterial meningitis, we examined the state of the major cortical antioxidant defenses in infant rats infected with Streptococcus pneumoniae. Adenine nucleotides were co-determined to assess possible changes in energy metabolism. Arguing against heightened parenchymal oxidant production, the high NADPH/NADP(+) ratio ( approximately 3:1) and activities of the major antioxidant defense and pentose phosphate pathway enzymes remained unchanged at the time of fulminant meningitis. In contrast, cortical ATP, ADP and total adenine nucleotides were on average decreased by approximately 25%. However, energy depletion did not lead to a significant decrease in adenylate energy charge (AEC). ATP depletion was likely a consequence of metabolic degradation, since it correlated with both the loss of total adenine nucleotides and accumulation of purine degradation products. Furthermore, the loss of ATP and decrease in AEC correlated significantly with the extent of neuronal injury. These results strongly suggest that energy depletion rather than parenchymal oxidative damage is involved in the observed cortical neuronal injury.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases
04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Infectiology

UniBE Contributor:

Leib, Stephen; Täuber, Martin G. and Christen, Stephan

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0006-8993

Publisher:

Elsevier

Language:

English

Submitter:

Stephen Leib

Date Deposited:

01 Sep 2014 08:07

Last Modified:

08 Jul 2015 07:57

Publisher DOI:

10.1016/S0006-8993(03)02557-5

PubMed ID:

12763248

Web of Science ID:

000183445900001

BORIS DOI:

10.7892/boris.52743

URI:

https://boris.unibe.ch/id/eprint/52743 (FactScience: 43472)

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