TILLING for Mutations in Model Plants and Crops

Tadele, Zerihun; Chikelu, MBA; Bradley, J. Till (2010). TILLING for Mutations in Model Plants and Crops. In: Jain, S. Mohan; Brar, D.S. (eds.) Molecular Techniques in Crop Improvement (pp. 307-332). Heidelberg: Springer Verlag 10.1007/978-90-481-2967-6_13

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A growing world population, changing climate and limiting fossil fuels will provide new pressures on human production of food, medicine, fuels and feed stock in the twenty-first century. Enhanced crop production promises to ameliorate these pressures. Crops can be bred for increased yields of calories, starch, nutrients, natural medicinal compounds, and other important products. Enhanced resistance to biotic and abiotic stresses can be introduced, toxins removed, and industrial qualities such as fibre strength and biofuel per mass can be increased. Induced and natural mutations provide a powerful method for the generation of heritable enhanced traits. While mainly exploited in forward, phenotype driven, approaches, the rapid accumulation of plant genomic sequence information and hypotheses regarding gene function allows the use of mutations in reverse genetic approaches to identify lesions in specific target genes. Such gene-driven approaches promise to speed up the process of creating novel phenotypes, and can enable the generation of phenotypes unobtainable by traditional forward methods. TILLING (Targeting Induced Local Lesions IN Genome) is a high-throughput and low cost reverse genetic method for the discovery of induced mutations. The method has been modified for the identification of natural nucleotide polymorphisms, a process called Ecotilling. The methods are general and have been applied to many species, including a variety of different crops. In this chapter the current status of the TILLING and Ecotilling methods and provide an overview of progress in applying these methods to different plant species, with a focus on work related to food production for developing nations.

Item Type:

Book Section (Book Chapter)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant Development
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Tadele, Zerihun

Subjects:

500 Science > 580 Plants (Botany)

ISBN:

978-90-481-2966-9

Publisher:

Springer Verlag

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

04 Oct 2013 14:17

Last Modified:

04 Jun 2014 16:41

Publisher DOI:

10.1007/978-90-481-2967-6_13

URI:

https://boris.unibe.ch/id/eprint/5315 (FactScience: 210055)

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