A novel family of transporters mediating the transport of glutathione derivatives in plants

Zhang, Ming-Yong; Bourbouloux, Andrée; Cagnac, Olivier; Srikanth, Chittur V.; Rentsch, Doris; Bachhawat, Anand K.; Delrot, Serge (2004). A novel family of transporters mediating the transport of glutathione derivatives in plants. Plant Physiology, 134(1), pp. 482-491. American Society of Plant Physiologists 10.1104/pp.103.030940

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Uptake and compartmentation of reduced glutathione (GSH), oxidized glutathione (GSSG), and glutathione conjugates are important for many functions including sulfur transport, resistance against biotic and abiotic stresses, and developmental processes. Complementation of a yeast (Saccharomyces cerevisiae) mutant (hgt1) deficient in glutathione transport was used to characterize a glutathione transporter cDNA (OsGT1) from rice (Oryza sativa). The 2.58-kb full-length cDNA (AF393848, gi 27497095), which was obtained by screening of a cDNA library and 5'-rapid amplification of cDNA ends-polymerase chain reaction, contains an open reading frame encoding a 766-amino acid protein. Complementation of the hgt1 yeast mutant strain with the OsGT1 cDNA restored growth on a medium containing GSH as the sole sulfur source. The strain expressing OsGT1 mediated H-3]GSH uptake, and this uptake was significantly competed not only by unlabeled GSSG and GS conjugates but also by some amino acids and peptides, suggesting a wide substrate specificity. OsGT1 may be involved in the retrieval of GSSG, GS conjugates, and nitrogen-containing peptides from the cell wall.

Item Type:	Journal Article (Original Article)
Division/Institute:	08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Molecular Plant Physiology 08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)
UniBE Contributor:	Rentsch, Doris
Subjects:	500 Science > 580 Plants (Botany)
ISSN:	0032-0889
Publisher:	American Society of Plant Physiologists
Language:	English
Submitter:	Peter Alfred von Ballmoos-Haas
Date Deposited:	06 Jun 2014 13:30
Last Modified:	05 Dec 2022 14:34
Publisher DOI:	10.1104/pp.103.030940
URI:	https://boris.unibe.ch/id/eprint/53321