The orf13 T-DNA gene of Agrobacterium rhizogenes confers meristematic competence to differentiated cells

Stieger, Pia A.; Meyer, Alain D.; Kathmann, Petra; Fründt, Corinne; Niederhauser, Isabel; Barone, Mario; Kuhlemeier, Cris (2004). The orf13 T-DNA gene of Agrobacterium rhizogenes confers meristematic competence to differentiated cells. Plant Physiology, 135(3), pp. 1798-1808. American Society of Plant Physiologists 10.1104/pp.104.040899

[img] Text
Kuhlemeier_Stieger.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (540kB) | Request a copy

Plant infections by the soil bacterium Agrobacterium rhizogenes result in neoplastic disease with the formation of hairy roots at the site of infection. Expression of a set of oncogenes residing on the stably integrated T-DNA is responsible for the disease symptoms. Besides the rol (root locus) genes, which are essential for the formation of hairy roots, the open reading frame orf13 mediates cytokinin-like effects, suggesting an interaction with hormone signaling pathways. Here we show that ORF13 induced ectopic expression of KNOX (KNOTTED1-like homeobox) class transcription factors, as well as of several genes involved in cell cycle control in tomato (Lycopersicon esculentum). ORF13 has a retinoblastoma (RB)-binding motif and interacted with maize (Zea mays) RB in vitro, whereas ORF13, bearing a point mutation in the RB-binding motif (ORF13*), did not. Increased cell divisions in the vegetative shoot apical meristem and accelerated formation of leaf primordia were observed in plants expressing orf13, whereas the expression of orf13* had no influence on cell division rates in the shoot apical meristem, suggesting a role of RB in the regulation of the cell cycle in meristematic tissues. On the other hand, ectopic expression of LeT6 was not dependent on a functional RB-binding motif. Hormone homeostasis was only altered in explants of leaves, whereas in the root no effects were observed. We suggest that ORF13 confers meristematic competence to cells infected by A. rhizogenes by inducing the expression of KNOX genes and promotes the transition of infected cells from the G1 to the S phase by binding to RB.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant Development

UniBE Contributor:

Stieger, Pia; Barone, Mario and Kuhlemeier, Cris

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0032-0889

Publisher:

American Society of Plant Physiologists

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

15 Aug 2014 12:10

Last Modified:

09 Oct 2015 15:13

Publisher DOI:

10.1104/pp.104.040899

BORIS DOI:

10.7892/boris.53806

URI:

https://boris.unibe.ch/id/eprint/53806

Actions (login required)

Edit item Edit item
Provide Feedback