Domains of expansin gene expression define growth regions in the shoot apex of tomato

Vogler, Hannes; Caderas, Doina; Mandel, Therese; Kuhlemeier, Cris (2003). Domains of expansin gene expression define growth regions in the shoot apex of tomato. Plant molecular biology, 53(3), pp. 267-272. Kluwer Academic Publishers 10.1023/B:PLAN.0000006999.48516.be

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Expansins are members of a multigene family of extracellular proteins, which increase cell wall extensibility in vitro and thus are thought to be involved in cell expansion. The major significance of the presence of this large gene family may be that distinctly expressed genes can independently regulate cell expansion in place and time. Here we report on LeExp9, a new expansin gene from tomato, and compare its expression in the shoot tip with that of LeExp2 and LeExp18. LeExp18 gene is expressed in very young tissues of the tomato shoot apex and the transcript levels are upregulated in the incipient primordium. LeExp2 mRNA accumulated in more mature tissues and transcript levels correlated with cell elongation in the elongation zone. In situ hybridization experiments showed a uniform distribution of LeExp9 mRNA in submeristematic tissues. When gibberellin-deficient mutant tomatoes that lacked elongation of the internodes were treated with gibberellin, the phenotypic rescue was correlated with an increase in LeExp9 and LeExp2, but not LeExp18 levels. We propose that the three expansins define three distinct growing zones in the shoot tip. In the meristem proper, gibberellin-independent LeExp18 mediates the cell expansion that accompanies cell division. In the submeristematic zone, LeExp9 mediates cell expansion at a time that cell division comes to a halt. LeExp9 expression requires gibberellin but the hormone is not normally limiting. Finally, LeExp2 mediates cell elongation in young stem tissue. LeExp2 expression is limited by the available gibberellin. These data suggest that regulation of cell wall extensibility is controlled, at least in part, by differential regulation of expansin genes.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant Development

UniBE Contributor:

Mandel, Therese and Kuhlemeier, Cris

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0167-4412

Publisher:

Kluwer Academic Publishers

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

15 Aug 2014 13:38

Last Modified:

26 Jun 2018 14:27

Publisher DOI:

10.1023/B:PLAN.0000006999.48516.be

Uncontrolled Keywords:

expansin, gibberellin, growth, shoot apex

BORIS DOI:

10.7892/boris.53808

URI:

https://boris.unibe.ch/id/eprint/53808

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