Microsurgical and laser ablation analysis of interactions between the zones and layers of the tomato shoot apical meristern

Reinhardt, Didier; Frenz, Martin; Mandel, Therese; Kuhlemeier, Cris (2003). Microsurgical and laser ablation analysis of interactions between the zones and layers of the tomato shoot apical meristern. Development, 130(17), pp. 4073-4083. Company of Biologists Limited 10.1242/dev.00596

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Plants exhibit life-long organogenic and histogenic activity in a specialised organ, the shoot apical meristem. Leaves and flowers are formed within the ring-shaped peripheral zone, which surrounds the central zone, the site of the stem cells. We have undertaken a series of high-precision laser ablation and microsurgical tissue removal experiments to test the functions of different parts of the tomato meristem, and to reveal their interactions. Ablation of the central zone led to ectopic expression of the WUSCHEL gene at the periphery, followed by the establishment of a new meristem centre. After the ablation of the central zone, organ formation continued without a lag. Thus, the central zone does not participate in organogenesis, except as the ultimate source of founder cells. Microsurgical removal of the external L-1 layer induced periclinal cell divisions and terminal differentiation in the subtending layers. In addition, no organs were initiated in areas devoid of L-1, demonstrating an important role of the L-1 in organogenesis. L-1 ablation had only local effects, an observation that is difficult to reconcile with phyllotaxis theories that invoke physical tension operating within the meristem as a whole. Finally, regeneration of L-1 cells was never observed after ablation. This shows that while the zones of the meristem show a remarkable capacity to regenerate after interference, elimination of the L-1 layer is irreparable and causes terminal differentiation.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)
08 Faculty of Science > Institute of Applied Physics
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant Development

UniBE Contributor:

Reinhardt, Didier; Frenz, Martin; Mandel, Therese and Kuhlemeier, Cris

Subjects:

500 Science > 580 Plants (Botany)
600 Technology > 620 Engineering

ISSN:

0950-1991

Publisher:

Company of Biologists Limited

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

15 Aug 2014 11:52

Last Modified:

25 Dec 2014 23:36

Publisher DOI:

10.1242/dev.00596

Uncontrolled Keywords:

laser ablation, meristem, WUSCHEL, central zone, peripheral zone, meristern layer, L1 layer, Lycopersicon esculentum

BORIS DOI:

10.7892/boris.53809

URI:

https://boris.unibe.ch/id/eprint/53809

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