The ethanolic fermentation pathway supports respiration and lipid biosynthesis in tobacco pollen

Mellema, Stefan; Eichenberger, Waldemar; Rawyler, André; Suter, Marianne; Tadege, Million; Kuhlemeier, Cris (2002). The ethanolic fermentation pathway supports respiration and lipid biosynthesis in tobacco pollen. The Plant Journal, 30(3), pp. 329-336. Blackwell Science 10.1046/j.1365-313X.2002.01293.x

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Rapid pollen tube growth requires a high rate of sugar metabolism to meet energetic and biosynthetic demands. Previous work on pollen sugar metabolism showed that tobacco pollen carry out efficient ethanolic fermentation concomitantly with a high rate of respiration (Bucher et al ., 1995). Here we show that the products of fermentation, acetaldehyde and ethanol, are further metabolised in a pathway that bypasses mitochondrial PDH. The enzymes involved in this pathway are pyruvate decarboxylase, aldehyde dehydrogenase and acetyl-CoA synthetase. Radiolabelling experiments show that during tobacco pollen tube growth label of C-14-ethanol is incorporated into CO2 as well as into lipids and other higher molecular weight compounds. A role for the glyoxylate cycle appears unlikely since activity of malate synthase, a key enzyme of the glyoxylate cycle, could not be detected.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Molecular Plant Physiology
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant Development

UniBE Contributor:

Suter, Marianne and Kuhlemeier, Cris

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0960-7412

Publisher:

Blackwell Science

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

15 Aug 2014 14:09

Last Modified:

24 Nov 2015 14:05

Publisher DOI:

10.1046/j.1365-313X.2002.01293.x

Uncontrolled Keywords:

fermentation; ethanol, PDH-bypass, ADH, tobacco pollen, sugar metabolism

BORIS DOI:

10.7892/boris.53815

URI:

https://boris.unibe.ch/id/eprint/53815

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