Optogenetic identification of a rapid eye movement sleep modulatory circuit in the hypothalamus.

Jego, Sonia; Glasgow, Stephen D.; Gutierrez Herrera, Carolina; Ekstrand, Mats; Reed, Sean J.; Boyce, Richard; Friedman, Jeffrey; Burdakov, Denis; Adamantidis, Antoine Roger (2013). Optogenetic identification of a rapid eye movement sleep modulatory circuit in the hypothalamus. Nature neuroscience, 16(11), pp. 1637-1643. Nature America 10.1038/nn.3522

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Rapid-eye movement (REM) sleep correlates with neuronal activity in the brainstem, basal forebrain and lateral hypothalamus. Lateral hypothalamus melanin-concentrating hormone (MCH)-expressing neurons are active during sleep, but their effects on REM sleep remain unclear. Using optogenetic tools in newly generated Tg(Pmch-cre) mice, we found that acute activation of MCH neurons (ChETA, SSFO) at the onset of REM sleep extended the duration of REM, but not non-REM, sleep episodes. In contrast, their acute silencing (eNpHR3.0, archaerhodopsin) reduced the frequency and amplitude of hippocampal theta rhythm without affecting REM sleep duration. In vitro activation of MCH neuron terminals induced GABAA-mediated inhibitory postsynaptic currents in wake-promoting histaminergic neurons of the tuberomammillary nucleus (TMN), and in vivo activation of MCH neuron terminals in TMN or medial septum also prolonged REM sleep episodes. Collectively, these results suggest that activation of MCH neurons maintains REM sleep, possibly through inhibition of arousal circuits in the mammalian brain.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Neurology

UniBE Contributor:

Gutierrez Herrera, Carolina and Adamantidis, Antoine Roger

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1097-6256

Publisher:

Nature America

Language:

English

Submitter:

Valentina Rossetti

Date Deposited:

18 Jun 2014 15:21

Last Modified:

29 Oct 2015 12:06

Publisher DOI:

10.1038/nn.3522

PubMed ID:

24056699

BORIS DOI:

10.7892/boris.53885

URI:

https://boris.unibe.ch/id/eprint/53885

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