Influence of high-conductivity buffer composition on field-enhanced sample injection coupled to sweeping in CE

Anres, Philippe; Delaunay, Nathalie; Vial, Jérôme; Thormann, Wolfgang; Gareil, Pierre (2013). Influence of high-conductivity buffer composition on field-enhanced sample injection coupled to sweeping in CE. Electrophoresis, 34(3), pp. 353-362. Wiley-VCH 10.1002/elps.201200374

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The aim of this work was to clarify the mechanism taking place in field-enhanced sample injection coupled to sweeping and micellar EKC (FESI-Sweep-MEKC), with the utilization of two acidic high-conductivity buffers (HCBs), phosphoric acid or sodium phosphate buffer, in view of maximizing sensitivity enhancements. Using cationic model compounds in acidic media, a chemometric approach and simulations with SIMUL5 were implemented. Experimental design first enabled to identify the significant factors and their potential interactions. Simulation demonstrates the formation of moving boundaries during sample injection, which originate at the initial sample/HCB and HCB/buffer discontinuities and gradually change the compositions of HCB and BGE. With sodium phosphate buffer, the HCB conductivity increased during the injection, leading to a more efficient preconcentration by staking (about 1.6 times) than with phosphoric acid alone, for which conductivity decreased during injection. For the same injection time at constant voltage, however, a lower amount of analytes was injected with sodium phosphate buffer than with phosphoric acid. Consequently sensitivity enhancements were lower for the whole FESI-Sweep-MEKC process. This is why, in order to maximize sensitivity enhancements, it is proposed to work with sodium phosphate buffer as HCB and to use constant current during sample injection.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Laboratory for Clinical Pharmacology
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases

UniBE Contributor:

Thormann, Wolfgang

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0173-0835

Publisher:

Wiley-VCH

Language:

English

Submitter:

Wolfgang Thormann

Date Deposited:

25 Jun 2014 16:22

Last Modified:

24 Jan 2015 01:19

Publisher DOI:

10.1002/elps.201200374

PubMed ID:

23161200

BORIS DOI:

10.7892/boris.53973

URI:

https://boris.unibe.ch/id/eprint/53973

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