NADPH oxidase-independent formation of extracellular DNA traps by basophils

Morshed, Md. Mahbubul; Hlushchuk, Ruslan; Simon, Dagmar; Walls, Andrew F.; Obata-Ninomiya, Kazushige; Karasuyama, Hajime; Djonov, Valentin; Eggel, Alexander; Kaufmann, Thomas; Simon, Hans-Uwe; Yousefi, Shida (2014). NADPH oxidase-independent formation of extracellular DNA traps by basophils. Journal of immunology, 192(11), pp. 5314-5323. American Association of Immunologists 10.4049/jimmunol.1303418

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Basophils are primarily associated with a proinflammatory and immunoregulatory role in allergic diseases and parasitic infections. Recent studies have shown that basophils can also bind various bacteria both in the presence and the absence of opsonizing Abs. In this report, we show that both human and mouse basophils are able to produce mitochondrial reactive oxygen species and to form extracellular DNA traps upon IL-3 priming and subsequent activation of the complement factor 5 a receptor or FcεRI. Such basophil extracellular traps (BETs) contain mitochondrial, but not nuclear DNA, as well as the granule proteins basogranulin and mouse mast cell protease 8. BET formation occurs despite the absence of any functional NADPH oxidase in basophils. BETs can be found in both human and mouse inflamed tissues, suggesting that they also play a role under in vivo inflammatory conditions. Taken together, these findings suggest that basophils exert direct innate immune effector functions in the extracellular space.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Institute for Immunology (discontinued)
04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Dermatology
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Pharmacology
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy

UniBE Contributor:

Morshed, Md. Mahbubul; Hlushchuk, Ruslan; Simon, Dagmar; Djonov, Valentin; Eggel, Alexander; Kaufmann, Thomas; Simon, Hans-Uwe and Yousefi, Shida

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0022-1767

Publisher:

American Association of Immunologists

Language:

English

Submitter:

Ruslan Hlushchuk

Date Deposited:

13 Oct 2014 10:29

Last Modified:

06 Nov 2015 10:49

Publisher DOI:

10.4049/jimmunol.1303418

PubMed ID:

24771850

BORIS DOI:

10.7892/boris.54047

URI:

https://boris.unibe.ch/id/eprint/54047

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