Germeyer, Ariane; Capp, Edison; Schlicksupp, Franziska; Jauckus, Julia; von Rango, Ulrike; von Wolff, Michael; Strowitzki, Thomas (2013). Cell-type specific expression and regulation of apolipoprotein D and E in human endometrium. European journal of obstetrics & gynecology and reproductive biology, 170(2), pp. 487-491. Elsevier 10.1016/j.ejogrb.2013.06.043
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OBJECTIVE
To assess the expression and regulation of antilipoprotein D (ApoD) and antilipoprotein E (ApoE) in human endometrium.
STUDY DESIGN
Endometrial biopsies from healthy, regularly cycling women were collected during the late proliferative and mid-secretory phase. mRNA gene expression of ApoD and ApoE was determined using real-time PCR in whole tissue, in isolated stromal (ESC), epithelial (EEC) and CD45(+) leukocytes (EIC), as well as after hormonal stimulation of ESC and EEC in vitro. Protein expression was analyzed using immunohistochemistry.
RESULTS
ApoD and ApoE mRNA was expressed in all cell types examined. A rise in ApoD mRNA expression was seen in whole endometrium, ESC, and EEC in the secretory phase, as well as after hormonal stimulation of ESC and EEC in vitro. ApoE mRNA was significantly upregulated in whole endometrium of secretory phase biopsies, while its expression was not altered by progesterone in vitro. Immunohistochemistry of whole endometrial tissue localized ApoD mainly in ESC and EEC. While ApoE was localized slightly in ESC, it was particularly noted on the surface of secretory phase endothelial cells.
CONCLUSION
We demonstrate for the first time the cell-type and cycle dependent expression of ApoD and ApoE within human endometrium, suggesting their role in endometrial modulation.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Gynaecology |
UniBE Contributor: |
von Wolff, Michael |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
0301-2115 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Nathalie Ursula Becher |
Date Deposited: |
26 Jun 2014 13:18 |
Last Modified: |
05 Dec 2022 14:35 |
Publisher DOI: |
10.1016/j.ejogrb.2013.06.043 |
PubMed ID: |
23895740 |
Uncontrolled Keywords: |
Apolipoprotein D, Apolipoprotein E, Endometrium Gene and protein expression |
BORIS DOI: |
10.7892/boris.54077 |
URI: |
https://boris.unibe.ch/id/eprint/54077 |
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