Intrapartum detection of Group B streptococci colonization by rapid PCR-test on labor ward

Müller, Andrea Martin; Henle, Ariane; Droz, Sara Christine; Kind, Andre B.; Rohner, Susanne; Baumann, Marc; Surbek, Daniel (2014). Intrapartum detection of Group B streptococci colonization by rapid PCR-test on labor ward. European journal of obstetrics & gynecology and reproductive biology, 176, pp. 137-141. Elsevier 10.1016/j.ejogrb.2014.02.039

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OBJECTIVE Group B streptococci (GBS) may lead to early onset neonatal sepsis with severe morbidity and mortality of newborns. Intrapartum detection of GBS is needed. The objective was to compare a PCR-based test performed in the laboratory versus labor ward. STUDY DESIGN 300 patients were included prospectively. In phase I, swabs were analyzed by selective culture and rapid PCR in the laboratory. In phase II, swabs were analyzed accordingly, but the PCR test was conducted in labor ward. Test performances were analyzed and compared. RESULTS In phase I the rapid PCR test had a sensitivity of 85.71% and a specificity of 95.9%. The GBS colonization rate was 18.67%. Overall 8.5% of the PCR results were invalid. In phase II the PCR test showed a sensitivity of 85.71% and a specificity of 95.65%. The GBS colonization rate was 23.3%. Overall 23.5% of swabs tested with PCR were invalid. Initiation of specific, short 2-hour training for operating personnel in the labor ward reduced the invalid test rate to 13.4%. CONCLUSION The rapid PCR-based test yields adequate results to identify GBS colonization when performed in labor ward. In order to reduce the number of invalid tests a short training period is needed.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases
04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Gynaecology

UniBE Contributor:

Müller, Andrea Martin; Droz, Sara Christine; Rohner, Susanne; Baumann, Marc and Surbek, Daniel


500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health








Nathalie Ursula Becher

Date Deposited:

13 Oct 2014 10:46

Last Modified:

11 Nov 2015 09:24

Publisher DOI:


PubMed ID:


Uncontrolled Keywords:

Group B streptococci, Neonatal sepsis




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