Transferrin immunoextraction for determination of carbohydrate-deficient transferrin in human serum by capillary zone electrophoresis

Caslavsky, Jitka; Joneli, Jeannine; Wanzenried, Ursula; Schiess, Jeannette; Thormann, Wolfgang (2012). Transferrin immunoextraction for determination of carbohydrate-deficient transferrin in human serum by capillary zone electrophoresis. Journal of separation science, 35(24), pp. 3521-3528. Wiley-VCH 10.1002/jssc.201200712

[img] Text
wt224-CDT extraction.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (723kB) | Request a copy

CZE-based assays for carbohydrate-deficient transferrin (CDT) in which serum is mixed with an Fe(III) ion-containing solution prior to analysis are effective approaches for the determination of CDT in patient samples. Sera of patients with progressed diseases, however, are prone to interferences comigrating with transferrin (Tf) that prevent the proper determination of CDT by CZE in these samples. The need of a simple and economic approach to immunoextract Tf from human serum prompted us to investigate the use of a laboratory-made anti-Tf spin column containing polyclonal rabbit anti-human Tf antibodies linked to Sepharose 4 Fast Flow beads. This article reports extraction column manufacturing and column characterization with sera having normal and elevated CDT levels. The developed procedure was applied to a number of relevant hepatology and dialysis patient samples and could thereby be shown to represent an effective method for extraction and concentration of all Tf isoforms. Furthermore, lipemic sera were delipidated using a mixture of diisopropyl ether and butanol prior to immunoextraction. CDT could unambiguously be determined in all pretreated samples.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Laboratory for Clinical Pharmacology

UniBE Contributor:

Caslavsky, Jitka; Joneli, Jeannine; Wanzenried, Ursula; Schiess, Jeannette and Thormann, Wolfgang

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1615-9306

Publisher:

Wiley-VCH

Language:

English

Submitter:

Wolfgang Thormann

Date Deposited:

04 Jul 2014 09:36

Last Modified:

07 Dec 2014 20:54

Publisher DOI:

10.1002/jssc.201200712

PubMed ID:

23109487

BORIS DOI:

10.7892/boris.54327

URI:

https://boris.unibe.ch/id/eprint/54327

Actions (login required)

Edit item Edit item
Provide Feedback