Efficient estimation of the total number of acini in adult rat lung

Barré, Sébastien; Haberthür, David; Stampanoni, Marco; Schittny, Johannes (2014). Efficient estimation of the total number of acini in adult rat lung. Physiological reports, 2(7), e12063. The American Physiological Society 10.14814/phy2.12063

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Pulmonary airways are subdivided into conducting and gas-exchanging airways. An acinus is defined as the small tree of gas-exchanging airways, which is fed by the most distal purely conducting airway. Until now a dissector of five consecutive sections or airway casts were used to count acini. We developed a faster method to estimate the number of acini in young adult rats. Right middle lung lobes were critical point dried or paraffin embedded after heavy metal staining and imaged by X-ray micro-CT or synchrotron radiation-based X-rays tomographic microscopy. The entrances of the acini were counted in three-dimensional (3D) stacks of images by scrolling through them and using morphological criteria (airway wall thickness and appearance of alveoli). Segmentation stopper were placed at the acinar entrances for 3D visualizations of the conducting airways. We observed that acinar airways start at various generations and that one transitional bronchiole may serve more than one acinus. A mean of 5612 (±547) acini per lung and a mean airspace volume of 0.907 (±0.108) μL per acinus were estimated. In 60-day-old rats neither the number of acini nor the mean acinar volume did correlate with the body weight or the lung volume.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Functional Anatomy
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy

UniBE Contributor:

Barré, Sébastien; Haberthür, David and Schittny, Johannes

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

2051-817X

Publisher:

The American Physiological Society

Language:

English

Submitter:

Johannes Schittny

Date Deposited:

13 Oct 2014 11:47

Last Modified:

16 Dec 2014 20:52

Publisher DOI:

10.14814/phy2.12063

PubMed ID:

24997068

Uncontrolled Keywords:

lung, pulmonary acinus, stereological analysis, X‐ray, microcomputed tomography, X‐ray tomographic microscopy

BORIS DOI:

10.7892/boris.54407

URI:

https://boris.unibe.ch/id/eprint/54407

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